LOCUS       sequence-92023-e       15456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses eNpHR 3.0-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15456)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRVdG-4Halo3-mCherry
FEATURES             Location/Qualifiers
     source          1..15456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5637..6341
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5779..5797)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6047..6066
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             8117..8128
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13128..13146)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13142..13189
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13212..13228)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13212..13228)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(13265..13285)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13265..13283)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13304..13320)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13304..13320)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13317..13339)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13328..13344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13352..13358,13359..13376,13377..13382))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13397..13418
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13535..13552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13706..14294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13786..13805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14465..15256,15257..15325))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15088..15107
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15326..15430)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 ggaaagccgc caccatgaca gagaccctgc ctcccgtgac cgagagtgcc gtggcccttc
     4741 aagccgaggt tacccaaagg gagttgttcg agttcgtgct gaacgaccct ttgcttgcaa
     4801 gcagtctcta tatcaacatc gcacttgcag gactgagtat actgctgttc gtttttatga
     4861 cccgaggact cgatgatcca cgggcaaaac ttattgctgt gtcaaccatc cttgtgcctg
     4921 tcgtcagcat tgcctcctac actggattgg cgagcggcct gacaatttcc gttcttgaaa
     4981 tgccagcggg ccattttgca gaaggcagct cagtgatgct gggaggagaa gaggtagatg
     5041 gtgtagtcac catgtgggga cggtatctca cctgggcact ttccacgccc atgattctcc
     5101 tcgctctggg tctcctggcc ggaagcaatg ctacaaagct cttcacagct atcactttcg
     5161 atatcgctat gtgcgtgact ggccttgccg cggccctgac tacctcctcc cacctcatga
     5221 gatggttctg gtacgctatc agttgtgcat gctttctggt ggtcttgtat atcctgctgg
     5281 tggagtgggc acaggacgcc aaagccgcgg gaaccgctga catgttcaat accctgaagc
     5341 tgttgacagt agtgatgtgg ctggggtatc caattgtgtg ggctcttgga gtcgagggta
     5401 tcgcggtgtt gcccgttggg gtgacgagct ggggatattc tttcctggat atcgtggcaa
     5461 agtacatttt cgcattcttg ctcctgaact atctgacgtc aaacgaatct gtcgtgtccg
     5521 gcagcatttt ggatgttcca tctgcttctg ggaccccggc tgatgatgcg gccgccaaga
     5581 gcaggatcac cagcgagggc gagtacatcc ccctggacca gatcgacatc aacgtggtga
     5641 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     5701 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     5761 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     5821 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     5881 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     5941 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     6001 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     6061 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     6121 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     6181 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     6241 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     6301 agggccgcca ctctaccggc ggcatggacg agctgtacaa gttctgctac gagaacgagg
     6361 tgtaatgacc cgggttataa agtgctgggt catctaagct tttcagtcga gaaaaaaaca
     6421 ttagatcaga agaacaactg gcaacacttc tcaacctgag acttacttca agatgctcga
     6481 tcctggagag gtctatgatg accctattga cccaatcgag ttagaggctg aacccagagg
     6541 aacccccatt gtccccaaca tcttgaggaa ctctgactac aatctcaact ctcctttgat
     6601 agaagatcct gctagactaa tgttagaatg gttaaaaaca gggaatagac cttatcggat
     6661 gactctaaca gacaattgct ccaggtcttt cagagttttg aaagattatt tcaagaaggt
     6721 agatttgggt tctctcaagg tgggcggaat ggctgcacag tcaatgattt ctctctggtt
     6781 atatggtgcc cactctgaat ccaacaggag ccggagatgt ataacagact tggcccattt
     6841 ctattccaag tcgtccccca tagagaagct gttgaatctc acgctaggaa atagagggct
     6901 gagaatcccc ccagagggag tgttaagttg ccttgagagg gttgattatg ataatgcatt
     6961 tggaaggtat cttgccaaca cgtattcctc ttacttgttc ttccatgtaa tcaccttata
     7021 catgaacgcc ctagactggg atgaagaaaa gaccatccta gcattatgga aagatttaac
     7081 ctcagtggac atcgggaagg acttggtaaa gttcaaagac caaatatggg gactgctgat
     7141 cgtgacaaag gactttgttt actcccaaag ttccaattgt ctttttgaca gaaactacac
     7201 acttatgcta aaagatcttt tcttgtctcg cttcaactcc ttaatggtct tgctctctcc
     7261 cccagagccc cgatactcag atgacttgat atctcaacta tgccagctgt acattgctgg
     7321 ggatcaagtc ttgtctatgt gtggaaactc cggctatgaa gtcatcaaaa tattggagcc
     7381 atatgtcgtg aatagtttag tccagagagc agaaaagttt aggcctctca ttcattcctt
     7441 gggagacttt cctgtattta taaaagacaa ggtaagtcaa cttgaagaga cgttcggtcc
     7501 ctgtgcaaga aggttcttta gggctctgga tcaattcgac aacatacatg acttggtttt
     7561 tgtgtttggc tgttacaggc attgggggca cccatatata gattatcgaa agggtctgtc
     7621 aaaactatat gatcaggttc accttaaaaa aatgatagat aagtcctacc aggagtgctt
     7681 agcaagcgac ctagccagga ggatccttag atggggtttt gataagtact ccaagtggta
     7741 tctggattca agattcctag cccgagacca ccccttgact ccttatatca aaacccaaac
     7801 atggccaccc aaacatattg tagacttggt gggggataca tggcacaagc tcccgatcac
     7861 gcagatcttt gagattcctg aatcaatgga tccgtcagaa atattggatg acaaatcaca
     7921 ttctttcacc agaacgagac tagcttcttg gctgtcagaa aaccgagggg ggcctgttcc
     7981 tagcgaaaaa gttattatca cggccctgtc taagccgcct gtcaatcccc gagagtttct
     8041 gaggtctata gacctcggag gattgccaga tgaagacttg ataattggcc tcaagccaaa
     8101 ggaacgggaa ttgaagattg aaggtcgatt ctttgctcta atgtcatgga atctaagatt
     8161 gtattttgtc atcactgaaa aactcttggc caactacatc ttgccacttt ttgacgcgct
     8221 gactatgaca gacaacctga acaaggtgtt taaaaagctg atcgacaggg tcaccgggca
     8281 agggcttttg gactattcaa gggtcacata tgcatttcac ctggactatg aaaagtggaa
     8341 caaccatcaa agattagagt caacagagga tgtattttct gtcctagatc aagtgtttgg
     8401 attgaagaga gtgttttcta gaacacacga gttttttcaa aaggcctgga tctattattc
     8461 agacagatca gacctcatcg ggttacggga ggatcaaata tactgcttag atgcgtccaa
     8521 cggcccaacc tgttggaatg gccaggatgg cgggctagaa ggcttacggc agaagggctg
     8581 gagtctagtc agcttattga tgatagatag agaatctcaa atcaggaaca caagaaccaa
     8641 aatactagct caaggagaca accaggtttt atgtccgaca tacatgttgt cgccagggct
     8701 atctcaagag gggctcctct atgaattgga gagaatatca aggaatgcac tttcgatata
     8761 cagagccgtc gaggaagggg catctaagct agggctgatc atcaagaaag aagagaccat
     8821 gtgtagttat gacttcctca tctatggaaa aacccctttg tttagaggta acatattggt
     8881 gcctgagtcc aaaagatggg ccagagtctc ttgcgtctct aatgaccaaa tagtcaacct
     8941 cgccaatata atgtcgacag tgtccaccaa tgcgctaaca gtggcacaac actctcaatc
     9001 tttgatcaaa ccgatgaggg attttctgct catgtcagta caggcagtct ttcactacct
     9061 gctatttagc ccaatcttaa agggaagagt ttacaagatt ctgagcgctg aaggggagag
     9121 ctttctccta gccatgtcaa ggataatcta tctagatcct tctttgggag ggatatctgg
     9181 aatgtccctc ggaagattcc atatacgaca gttctcagac cctgtctctg aagggttatc
     9241 cttctggaga gagatctggt taagctccca agagtcctgg attcacgcgt tgtgtcaaga
     9301 ggctggaaac ccagatcttg gagagagaac actcgagagc ttcactcgcc ttctagaaga
     9361 tccgaccacc ttaaatatca gaggaggggc cagtcctacc attctactca aggatgcaat
     9421 cagaaaggct ttatatgacg aggtggacaa ggtggaaaat tcagagtttc gagaggcaat
     9481 cctgttgtcc aagacccata gagataattt tatactcttc ttaatatctg ttgagcctct
     9541 gtttcctcga tttctcagtg agctattcag ttcgtctttt ttgggaatcc ccgagtcaat
     9601 cattggattg atacaaaact cccgaacgat aagaaggcag tttagaaaga gtctctcaaa
     9661 aactttagaa gaatccttct acaactcaga gatccacggg attagtcgga tgacccagac
     9721 acctcagagg gttggggggg tgtggccttg ctcttcagag agggcagatc tacttaggga
     9781 gatctcttgg ggaagaaaag tggtaggcac gacagttcct cacccttctg agatgttggg
     9841 attacttccc aagtcctcta tttcttgcac ttgtggagca acaggaggag gcaatcctag
     9901 agtttctgta tcagtactcc cgtcctttga tcagtcattt ttttcacgag gccccctaaa
     9961 gggatacttg ggctcgtcca cctctatgtc gacccagcta ttccatgcat gggaaaaagt
    10021 cactaatgtt catgtggtga agagagctct atcgttaaaa gaatctataa actggttcat
    10081 tactagagat tccaacttgg ctcaagctct aattaggaac attatgtctc tgacaggccc
    10141 tgatttccct ctagaggagg cccctgtctt caaaaggacg gggtcagcct tgcataggtt
    10201 caagtctgcc agatacagcg aaggagggta ttcttctgtc tgcccgaacc tcctctctca
    10261 tatttctgtt agtacagaca ccatgtctga tttgacccaa gacgggaaga actacgattt
    10321 catgttccag ccattgatgc tttatgcaca gacatggaca tcagagctgg tacagagaga
    10381 cacaaggcta agagactcta cgtttcattg gcacctccga tgcaacaggt gtgtgagacc
    10441 cattgacgac gtgaccctgg agacctctca gatcttcgag tttccggatg tgtcgaaaag
    10501 aatatccaga atggtttctg gggctgtgcc tcacttccag aggcttcccg atatccgtct
    10561 gagaccagga gattttgaat ctctaagcgg tagagaaaag tctcaccata tcggatcagc
    10621 tcaggggctc ttatactcaa tcttagtggc aattcacgac tcaggataca atgatggaac
    10681 catcttccct gtcaacatat acggcaaggt ttcccctaga gactatttga gagggctcgc
    10741 aaggggagta ttgataggat cctcgatttg cttcttgaca agaatgacaa atatcaatat
    10801 taatagacct cttgaattgg tctcaggggt aatctcatat attctcctga ggctagataa
    10861 ccatccctcc ttgtacataa tgctcagaga accgtctctt agaggagaga tattttctat
    10921 ccctcagaaa atccccgccg cttatccaac cactatgaaa gaaggcaaca gatcaatctt
    10981 gtgttatctc caacatgtgc tacgctatga gcgagagata atcacggcgt ctccagagaa
    11041 tgactggcta tggatctttt cagactttag aagtgccaaa atgacgtacc tatccctcat
    11101 tacttaccag tctcatcttc tactccagag ggttgagaga aacctatcta agagtatgag
    11161 agataacctg cgacaattga gttctttgat gaggcaggtg ctgggcgggc acggagaaga
    11221 taccttagag tcagacgaca acattcaacg actgctaaaa gactctttac gaaggacaag
    11281 atgggtggat caagaggtgc gccatgcagc tagaaccatg actggagatt acagccccaa
    11341 caagaaggtg tcccgtaagg taggatgttc agaatgggtc tgctctgctc aacaggttgc
    11401 agtctctacc tcagcaaacc cggcccctgt ctcggagctt gacataaggg ccctctctaa
    11461 gaggttccag aaccctttga tctcgggctt gagagtggtt cagtgggcaa ccggtgctca
    11521 ttataagctt aagcctattc tagatgatct caatgttttc ccatctctct gccttgtagt
    11581 tggggacggg tcagggggga tatcaagggc agtcctcaac atgtttccag atgccaagct
    11641 tgtgttcaac agtcttttag aggtgaatga cctgatggct tccggaacac atccactgcc
    11701 tccttcagca atcatgaggg gaggaaatga tatcgtctcc agagtgatag atcttgactc
    11761 aatctgggaa aaaccgtccg acttgagaaa cttggcaacc tggaaatact tccagtcagt
    11821 ccaaaagcag gtcaacatgt cctatgacct cattatttgc gatgcagaag ttactgacat
    11881 tgcatctatc aaccggatca ccctgttaat gtccgatttt gcattgtcta tagatggacc
    11941 actctatttg gtcttcaaaa cttatgggac tatgctagta aatccaaact acaaggctat
    12001 tcaacacctg tcaagagcgt tcccctcggt cacagggttt atcacccaag taacttcgtc
    12061 tttttcatct gagctctacc tccgattctc caaacgaggg aagtttttca gagatgctga
    12121 gtacttgacc tcttccaccc ttcgagaaat gagccttgtg ttattcaatt gtagcagccc
    12181 caagagtgag atgcagagag ctcgttcctt gaactatcag gatcttgtga gaggatttcc
    12241 tgaagaaatc atatcaaatc cttacaatga gatgatcata actctgattg acagtgatgt
    12301 agaatctttt ctagtccaca agatggttga tgatcttgag ttacagaggg gaactctgtc
    12361 taaagtggct atcattatag ccatcatgat agttttctcc aacagagtct tcaacgtttc
    12421 caaaccccta actgacccct cgttctatcc accgtctgat cccaaaatcc tgaggcactt
    12481 caacatatgt tgcagtacta tgatgtatct atctactgct ttaggtgacg tccctagctt
    12541 cgcaagactt cacgacctgt ataacagacc tataacttat tacttcagaa agcaagtcat
    12601 tcgagggaac gtttatctat cttggagttg gtccaacgac acctcagtgt tcaaaagggt
    12661 agcctgtaat tctagcctga gtctgtcatc tcactggatc aggttgattt acaagatagt
    12721 gaagactacc agactcgttg gcagcatcaa ggatctatcc agagaagtgg aaagacacct
    12781 tcataggtac aacaggtgga tcaccctaga ggatatcaga tctagatcat ccctactaga
    12841 ctacagttgc ctgtgaaccg gatactcctg gaagcctgcc catgctaaga ctcttgtgtg
    12901 atgtatcttg aaaaaaacaa gatcctaaat ctgaaccttt ggttgtttga ttgtttttct
    12961 catttttgtt gtttatttgt taagcgtggg tcggcatggc atctccacct cctcgcggtc
    13021 cgacctgggc atccgaagga ggacgcacgt ccactcggat ggctaaggga ggggcgggga
    13081 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
    13141 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
    13201 aactatatcc ggatccacta gttctagagc ggccgccacc gcggtggagc tccagctttt
    13261 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
    13321 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    13381 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    13441 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    13501 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    13561 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    13621 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    13681 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    13741 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    13801 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    13861 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    13921 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    13981 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    14041 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    14101 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    14161 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    14221 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    14281 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    14341 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    14401 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    14461 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    14521 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    14581 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    14641 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    14701 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    14761 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    14821 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    14881 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    14941 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    15001 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    15061 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    15121 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    15181 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    15241 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    15301 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    15361 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    15421 gggttccgcg cacatttccc cgaaaagtgc cacctg
//
