LOCUS       sequence-92047-e         400 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dre-Dependent ChR2-EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone
            J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H,
            Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, 
            Deisseroth K
  TITLE     Targeting cells with single vectors using multiple-feature Boolean 
            logic.
  JOURNAL   Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 
            2014 Jun 8.
  PUBMED    24908100
REFERENCE   2  (bases 1 to 400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2996"; journalName: "Nat Methods"; date: "2014-07"; 
            volume: "11"; issue: "7"; pages: "763-72"
COMMENT     Sequence Label: pAAV-Ef1a-dDIO hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..400
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..44
                     /label=rox
                     /bound_moiety="Dre recombinase"
     CDS             complement(141..392)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(183..204)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtcgactccg gataacttta aataatgcca attatttaaa gttagcagaa tggtagctgg
       61 attgtagctg ctattagcaa tatgaaacct cttataactt taaataatgt cgattattta
      121 aagttaggcg cgccctatta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
      181 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
      241 gactggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
      301 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
      361 aagttcacct tgatgccgtt cttctgcttg tcggccatga
//
