LOCUS       sequence-92055-e         430 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone
            J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H,
            Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, 
            Deisseroth K
  TITLE     Targeting cells with single vectors using multiple-feature Boolean 
            logic.
  JOURNAL   Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 
            2014 Jun 8.
  PUBMED    24908100
REFERENCE   2  (bases 1 to 430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2996"; journalName: "Nat Methods"; date: "2014-07"; 
            volume: "11"; issue: "7"; pages: "763-72"
COMMENT     Sequence Label: pAAV-nEF Con/Fon hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..430
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(100..121)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    234..267
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    318..351
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
ORIGIN
        1 gaggtcgaga ctctcgtcga agacgaagcc gaggccggag ccgtgccagc ggccgccgtg
       61 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      121 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      181 ctgaccctga agttcatctg caccaccggc aaggtgagta caggaggggt accataactt
      241 cgtataaagt atcctatacg aagttatttg ccttaaccca gaaattatca ctgttattct
      301 ttagaatggt gcaaagaata acttcgtata aggtatacta tacgaagtta tgctagcaac
      361 cccgacactt accggtgagg ttgctcaggc ggataaggat gacaggacaa gtgagcagcc
      421 actctgcata
//
