LOCUS       sequence-92062-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses constitutively active human PKCi with a deletion on the 
            PB1 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Forteza R, Wald FA, Mashukova A, Kozhekbaeva Z, Salas PJ
  TITLE     Par-complex aPKC and Par3 cross-talk with innate immunity NF-kappaB 
            pathway in epithelial cells.
  JOURNAL   Biol Open. 2013 Oct 8;2(11):1264-9. doi: 10.1242/bio.20135918. 
            eCollection 2013.
  PUBMED    24244864
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/bio.20135918"; journalName: "Biol Open"; date: "2013-10-8- 
            8"; volume: "2"; issue: "11"; pages: "1264-9"
COMMENT     Sequence Label: pLenti6.3/V5-DEST-PKCi-A120E-Delta-PB1
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    79..103
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgactagt ccagtgtggt
       61 ggaattctgc agatatcaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc
      121 accatgtccc acacggtcgc aggcggcggc agcggggacc attcccacca ggtccgggtg
      181 aaagcctact actgtgtacc agaacgtcct gggatgcctt gtccaggaga agataaatcc
      241 atctaccgta gaggtgaacg ccgctggaga aagctttatt gtgccaatgg ccacactttc
      301 caagccaagc gtttcaacag gcgtgctcac tgtgccatct gcacagaccg aatatgggga
      361 cttggacgcc aaggatataa gtgcatcaac tgcaaactct tggttcataa gaagtgccat
      421 aaactcgtca caattgaatg tgggcggcat tctttgccac aggaaccagt gatgcccatg
      481 gatcagtcat ccatgcattc tgaccatgca cagacagtaa ttccatataa tccttcaagt
      541 catgagagtt tggatcaagt tggtgaagaa aaagaggcaa tgaacaccag ggaaagtggc
      601 aaagcttcat ccagtctagg tcttcaggat tttgatttgc tccgggtaat aggaagagga
      661 agttatgcca aagtactgtt ggttcgatta aaaaaaacag atcgtattta tgcaatgaaa
      721 gttgtgaaaa aagagcttgt taatgatgat gaggatattg attgggtaca gacagagaag
      781 catgtgtttg agcaggcatc caatcatcct ttccttgttg ggctgcattc ttgctttca
//
