LOCUS       sequence-92072-e         307 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-on/Flp-off ChR2-EYFP under the short Ef1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone
            J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H,
            Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, 
            Deisseroth K
  TITLE     Targeting cells with single vectors using multiple-feature Boolean 
            logic.
  JOURNAL   Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 
            2014 Jun 8.
  PUBMED    24908100
REFERENCE   2  (bases 1 to 307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2996"; journalName: "Nat Methods"; date: "2014-07"; 
            volume: "11"; issue: "7"; pages: "763-72"
COMMENT     Sequence Label: pAAV-nEF Con/Foff hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..307
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(72..93)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    206..239
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ccgaggccgg agccgtgcca gcggccgccg tgagcaaggg cgaggagctg ttcaccgggg
       61 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      121 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      181 gcaaggtgag tacaggaggg gtaccataac ttcgtataaa gtatcctata cgaagttatt
      241 tgccttaacc cagaaattat cactgttatt ctttagaatg gtgcaaagaa taacttcgta
      301 taaggta
//
