LOCUS       sequence-92092-e        5307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of OleD (DQ195536) under arabinose promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5307)
  TITLE     Anderson Lab Glucosyltransferase plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBAD-OleD
FEATURES             Location/Qualifiers
     source          1..5307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1271..1300
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1316..1333
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1389..1406)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1559..1645
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1737..1764
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1784..1875
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1876..1944,1945..2736)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2094..2113)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2907..3495
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3396..3415
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3649..3666
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3681..3821
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3747..3766)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3907..3929
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4085..4963)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4990..5274
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     5197..5216
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 catggctacc acccagacca ctcccgccca catcgccatg ttctccatcg ccgcccacgg
       61 ccatgtgaac cccagcctgg aggtgatccg tgaactcgtc gcccgcggcc accgggtcac
      121 gtacgccatt ccgcccgtct tcgccgacaa ggtggccgcc accggcgccc ggcccgtcct
      181 ctaccactcc accctgcccg gccccgacgc cgacccggag gcatggggaa gcaccctgct
      241 ggacaacgtc gaaccgttcc tgaacgacgc gatccaggcg ctcccgcagc tcgccgatgc
      301 ctacgccgac gacatccccg atctcgtcct gcacgacatc acctcctacc cggcccgcgt
      361 cctggcccgc cgctggggcg tcccggcggt ctccctctcc ccgaacctcg tcgcctggaa
      421 gggttacgag gaggaggtcg ccgagccgat gtggcgcgaa ccccggcaga ccgagcgcgg
      481 acgggcctac tacgcccggt tcgaggcatg gctgaaggag aacgggatca ccgagcaccc
      541 ggacacgttc gccagtcatc cgccgcgctc cctggtgctc atcccgaagg cgctccagcc
      601 gcacgccgac cgggtggacg aagacgtgta caccttcgtc ggcgcctgcc agggagaccg
      661 cgccgaggaa ggcggctggc agcggcccgc cggcgcggag aaggtcgtcc tggtgtcgct
      721 cggctcggcg ttcaccaagc agcccgcctt ctaccgggag tgcgtgcgcg ccttcgggaa
      781 cctgcccggc tggcacctcg tcctccagat cggccggaag gtgacccccg ccgaactggg
      841 ggagctgccg gacaacgtgg aggtgcacga ctgggtgccg cagctcgcga tcctgcgcca
      901 ggccgatctg ttcgtcaccc acgcgggcgc cggcggcagc caggaggggc tggccaccgc
      961 gacgcccatg atcgccgtac cgcaggccgt cgaccagttc ggcaacgccg acatgctcca
     1021 agggctcggc gtcgcccgga agctggcgac cgaggaggcc accgccgacc tgctccgcga
     1081 gaccgccctc gctctggtgg acgacccgga ggtcgcgcgc cggctccggc gaatccaggc
     1141 ggagatggcc caggagggcg gcacccggcg ggcggccgac ctcatcgagg ccgaactgcc
     1201 cgcgcgccac gagcggcagg agccggtggg cgaccgaccc aacggtgggg ggaattcgaa
     1261 gcttgggccc gaacaaaaac tcatctcaga agaggatctg aatagcgccg tcgaccatca
     1321 tcatcatcat cattgagttt aaacggtctc cagcttggct gttttggcgg atgagagaag
     1381 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg
     1441 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
     1501 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca
     1561 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1621 gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
     1681 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa
     1741 ggccatcctg acggatggcc tttttgcgtt tctacaaact ctttttgttt atttttctaa
     1801 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     1861 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     1921 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     1981 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     2041 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     2101 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     2161 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     2221 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     2281 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     2341 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     2401 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     2461 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     2521 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     2581 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     2641 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     2701 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     2761 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     2821 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2881 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2941 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3001 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     3061 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3121 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3181 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3241 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3301 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3361 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3421 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3481 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3541 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3601 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3661 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3721 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3781 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     3841 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     3901 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     3961 agcagatcaa ttcgcgcgcg aaggcgaagc ggcatgcata atgtgcctgt caaatggacg
     4021 aagcagggat tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta
     4081 ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact
     4141 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa
     4201 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg
     4261 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga
     4321 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa
     4381 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca
     4441 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca
     4501 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc
     4561 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg
     4621 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc
     4681 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg
     4741 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc
     4801 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa
     4861 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa
     4921 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc
     4981 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt
     5041 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa
     5101 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag
     5161 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa
     5221 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt
     5281 ttttttgggc taacaggagg aattaac
//
