LOCUS       sequence-92096-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the pH sensor ratiometric pHluorin in the mitochondria of 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Venkatachalam V, Cohen AE
  TITLE     Imaging GFP-Based Reporters in Neurons with Multiwavelength 
            Optogenetic Control.
  JOURNAL   Biophys J. 2014 Oct 7;107(7):1554-63. doi: 
            10.1016/j.bpj.2014.08.020.
  PUBMED    25296307
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2014.08"; journalName: "Biophys J"; date: "2014-10-7-
            7"; volume: "107"; issue: "7"; pages: "1554-63"
COMMENT     Sequence Label: VV064: 1xCox8 - ratiometric pHluorin in fck
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(89..109)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattcatt tgtatagttc
       61 atccatgcca tgtgtaatcc cagcagctgt tacaaactca agaaggacca tgtggtctct
      121 cttttcgttg ggatctttcg aaagggcaga ttgtgtgtgc aggtaatggt tgtctggtaa
      181 aaggacaggg ccatcgccaa ttggagtatt ttgttgataa tggtctgcta gttgaacgcc
      241 tccatcttca atgttgtggt gaacttgaaa gatagctttg gtaccattct tttgtttgtc
      301 tgccatgatg tacaccaagt gctcgttata gttgtattcc aatttgtgtc caagaatgtt
      361 tccatcatct ttaaaatcaa taccttttaa ctcgattcta ttaacaaggg tatcaccttc
      421 aaacttgact tcagcacgtg tcttgtagtt cccgtcatct ttgaaaaata tagttctttc
      481 ctgtacataa ccttcgggca tggcactctt gaaaaagtca tgccgtttca tatgatctgg
      541 gtatcttgaa aagcattgaa caccataaga gaaagtagtg acaagtgttg gccatggaac
      601 aggtagtttt ccagtagtgc aaataaattt aagggtaagt tttccgtatg ttgcatcacc
      661 ttcaccctct ccactgacag aaaatttgtg cccattaaca tcaccatcta attcaacaag
      721 aattggg
//
