LOCUS       sequence-92101-e         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the calcium sensor GCaMP5G in the mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Venkatachalam V, Cohen AE
  TITLE     Imaging GFP-Based Reporters in Neurons with Multiwavelength 
            Optogenetic Control.
  JOURNAL   Biophys J. 2014 Oct 7;107(7):1554-63. doi: 
            10.1016/j.bpj.2014.08.020.
  PUBMED    25296307
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2014.08"; journalName: "Biophys J"; date: "2014-10-7-
            7"; volume: "107"; issue: "7"; pages: "1554-63"
COMMENT     Sequence Label: VV073: 1xCox8 - GCaMP5G in fubi
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..491)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     607..626
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattctca cttcgctgtc
       61 atcatttgta caaactcttc gtagtttacc tgaccatccc catcgatgtc tgcttccctg
      121 atcatttcat caacctcttc atctgttaac ttctctccaa ggtttgtcat cacgtggcga
      181 agctctgctg cactgatgta gccattgcca tccttatcaa acacacggaa cgcttctcta
      241 atttcttctt cactgtctgt gtatttcatt tttcttgcca tcattgtcag gaactcaggg
      301 aagtcgattg tgccgtcacc gtcggcatct acttcattga tcatgtcctg cagctctgct
      361 tctgtggggt tctgccccag agaccgcatc accgtcccca gctccttggt tgttattgtc
      421 ccatccccgt ccttgtcaaa tagggagaaa gcctctttaa attctgcgat ctgctcttca
      481 gtcagttggt ccggcaggtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      541 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      601 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gatgtagcct
      661 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaa
//
