LOCUS       sequence-92105-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CoChR with the C108S (step function opsin) mutation fused 
            to eGFP under the a-CamKII promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Venkatachalam V, Cohen AE
  TITLE     Imaging GFP-Based Reporters in Neurons with Multiwavelength 
            Optogenetic Control.
  JOURNAL   Biophys J. 2014 Oct 7;107(7):1554-63. doi: 
            10.1016/j.bpj.2014.08.020.
  PUBMED    25296307
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2014.08"; journalName: "Biophys J"; date: "2014-10-7-
            7"; volume: "107"; issue: "7"; pages: "1554-63"
COMMENT     Sequence Label: VV220: CoChR(C108S)-eGFP in fck
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(49..762,763..765))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(52..762,763..765))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(94..115)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     443..462
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     702..723
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattctt acttgtacag
       61 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
      121 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
      181 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
      241 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
      301 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
      361 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
      421 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
      481 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
      541 ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg
      601 cacgggcagc ttgccggtgg tgcaaatgaa cttcagggtc agcttgccgt aggtggcatc
      661 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
      721 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcactgcta ctaccggtgc
      781 cgccactgtc tcctcgtcct cctggtccac catggtctcc act
//
