LOCUS       sequence-92116-e         605 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized S. pyogenes Cas9 from the human 
            PGK-1 gene promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Maggio I, Holkers M, Liu J, Janssen JM, Chen X, Goncalves MA
  TITLE     Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease 
            complexes induces targeted mutagenesis in a diverse array of human 
            cells.
  JOURNAL   Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105.
  PUBMED    24870050
REFERENCE   2  (bases 1 to 605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-05-29"; volume: "4:5105. doi"; pages: " 
            10.1038/srep05105"
COMMENT     Sequence Label: pAdSh.PGK.Cas9
FEATURES             Location/Qualifiers
     source          1..605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
       61 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatct
      121 gggcgtggtt aagggtggga aagaatatat aaggtggggg tcttatgtag ttttgtatct
      181 gttttgcagc agccgccgcc gccatgagca ccaactcgtt tgatggaagc attgtgagct
      241 catatttgac aacgcgcatg cccccatggg ccggggtgcg tcagaatgtg atgggctcca
      301 gcattgatgg tcgccccgtc ctgcccgcaa actctactac cttgacctac gagaccgtgt
      361 ctggaacgcc gttggagact gcagcctccg ccgccgcttc agccgctgca gccaccgccc
      421 gcgggattgt gactgacttt gctttcctga gcccgcttgc aagcagtgca gcttcccgtt
      481 catccgcccg cgatgacaag ttgacggctc ttttggcaca attggattct ttgacccggg
      541 aacttaatgt cgtttctcag cagctgttgg atctgcgcca gcaggtttct gccctgaagg
      601 cttcc
//
