LOCUS       sequence-92119-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 promoter-driven single guide RNA complementary to eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Maggio I, Holkers M, Liu J, Janssen JM, Chen X, Goncalves MA
  TITLE     Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease 
            complexes induces targeted mutagenesis in a diverse array of human 
            cells.
  JOURNAL   Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105.
  PUBMED    24870050
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-05-29"; volume: "4:5105. doi"; pages: " 
            10.1038/srep05105"
COMMENT     Sequence Label: pAdSh.U6.gRNAGFP
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    52..76
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(88..163)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(184..432)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(242..261)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(412..432)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_signal     623..773
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
ORIGIN
        1 agcggccgct cgagctctgt acatgtccgc ggtcgaattc gccctttaat gccaactttg
       61 tacaagaaag ctgggtctag aaaaaaagca ccgactcggt gccacttttt caagttgata
      121 acggactagc cttattttaa cttgctattt ctagctctaa aactgaagaa gatggtgcgc
      181 tccggtgttt cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt
      241 tacggtaagc atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag
      301 aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt
      361 aattctaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg
      421 aaataggccc tcttcctgcc cgaccttggt accggatcca gtcgactgaa ttggttcctt
      481 taaagcctgc ttttttgtac aaagggcgaa ttcgacgtac gcgtatcgat ggcgccagct
      541 gcaggcggcc gccatatgca tcctaggcct attaatattc cggagtatac gtagccggct
      601 aacgttaaca accggtacca gtattacgcg ctatgagtaa cacaaaatta ttcagatttc
      661 acttcctctt attcagtttt cccgcgaaaa tggccaaatc ttactcggtt acgcccaaat
      721 ttactacaac atccgcctaa aaccgcgcga aaattgtcac ttcctgtgta caccggcgca
      781 caccaaaaa
//
