LOCUS       sequence-92143-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Segarra M, Vilardell C, Matsumoto K, Esparza J, Lozano E, 
            Serra-Pages C, Urbano-Marquez A, Yamada KM, Cid MC
  TITLE     Dual function of focal adhesion kinase in regulating 
            integrin-induced MMP-2 and MMP-9 release by human T lymphoid cells.
  JOURNAL   FASEB J. 2005 Nov;19(13):1875-7.
  PUBMED    16260653
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J."; 
            date: "2005-11"; volume: "19(13)"; pages: "1875-7"
COMMENT     Sequence Label: pRKVSV FRNK
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     239..256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     270..289
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      335..344
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             347..379
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             380..412
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc attggaacgc
      121 ggattccccg tgccaagagt gacgtaagta ccgcctatag agtctatagg cccaccccct
      181 tggcttcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
      241 ttaggtgaca ctatagaata acatccactt tgcctttctc tccacaggtg tccactccca
      301 ggtccaactg cacctcggtt ctatcgattc agccgccacc atgggataca ccgacatcga
      361 gatgaaccgg ctgggcaagt acaccgacat cgagatgaac cggctgggca agggatccat
      421 ggaatccaga agacaggcta ccgtgtcctg ggactctgga gggtctgatg aagcaccacc
      481 caagcccagc agacctggtt atcctagccc gagatccagt gaaggatttt atcccagccc
      541 acagcacatg gtccagacca atcactacca ggtctctggc tacccgggtt cccatggaat
      601 cccagccatg gctggcagca tttaccaagg tcaggcatct cttctggacc agacagaatt
      661 gtggaatcac agacctcagg agatgtccat gtggcagccc agtgtggagg attcggcagc
      721 tttggacctt cgagggatgg gacaggtgct tccccctcac ctgatggaag agcggctaat
      781 ccgacagcag caggaaatgg aagaagacca gcgctggctg gagaaggaag agcgattcct
      841 gaaacctgat gtgaggctct ctcgaggcag catcgacagg gaagacggga gttttcaggg
      901 tccgactgga aaccaacaca tctatcagcc tgtggggaag ccagatcctg cagctccacc
      961 aaagaaacct cctcgccctg gagcacctgg ccacctaagc aacctgtcca gcatcagcag
     1021 ccc
//
