LOCUS       sequence-92145-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Lopez ME, Klein AD, Dimbil UJ, Scott MP
  TITLE     Anatomically defined neuron-based rescue of neurodegenerative 
            Niemann-Pick type C disorder.
  JOURNAL   J Neurosci. 2011 Mar 23;31(12):4367-78. doi: 
            10.1523/JNEUROSCI.5981-10.2011.
  PUBMED    21430138
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.5981-10"; journalName: "J Neurosci"; date: 
            "2011-03-23- 23"; volume: "31"; issue: "12"; pages: "4367-78"
COMMENT     Sequence Label: NPC1 His6 ECFP
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(103..615,616..618,619..621))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     296..315
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     555..576
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      618..627
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(652..669)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
       61 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
      121 cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga cgttgtggct
      181 gatgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
      241 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
      301 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
      361 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc
      421 ccaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
      481 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
      541 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
      601 ctcctcgccc ttgctcacca tggtggcgat ggatcccggg cccgcggtac cgtggtggtg
      661 gtggtggtga aaattgagga gccgttctct ctctgtccct ctgtagcgct cgtaagtggt
      721 gtgtctttta gctttattca ccgacggccc tatgtaactg agtaagacgg gaagaaatat
      781 tagtccatga gtggctccga gtaagaccat ggctaagtac atcctgaagt aa
//
