LOCUS       sequence-92149-e         457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-Dependent Chloride Channel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Berndt A, Lee SY, Ramakrishnan C, Deisseroth K
  TITLE     Structure-guided transformation of channelrhodopsin into a 
            light-activated chloride channel.
  JOURNAL   Science. 2014 Apr 25;344(6182):420-4. doi: 10.1126/science.1252367.
  PUBMED    24763591
REFERENCE   2  (bases 1 to 457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-04-25- 25"; volume: "344"; 
            issue: "6182"; pages: "420-4"
COMMENT     Sequence Label: pAAV-Ef1a-DIO SwiChRca-TS-EYFP
FEATURES             Location/Qualifiers
     source          1..457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(21..54)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(105..138)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(153..404)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(195..216)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cctctagagt cgactccgga ataacttcgt ataggatact ttatacgaag ttatgcagaa
       61 tggtagctgg attgtagctg ctattagcaa tatgaaacct cttaataact tcgtatagca
      121 tacattatac gaagttatgg cgcgccctat tacttgtaca gctcgtccat gccgagagtg
      181 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
      241 ttgctcaggg cggactggta gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
      301 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
      361 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
      421 ttgtggctgt tgtagttgta ctccagcttg tgcccca
//
