LOCUS       sequence-92150-e        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the
            linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI 
            /ClaI). Plasmid confers ampicillin resistance to DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: p416GAL1-Zipp.-L-IFP-F[1] (Yeast)
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(457..513)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     promoter        complement(606..1047)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(612..635)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 gagttaccag gcctcggtag gttcgaactc caggatcagg agttcagcca cccgatgcac
       61 agtcaggctg aggtgcccag cagctggcca gtcgagggtg gccctgtatt ggagggcatc
      121 tggacatcct gggggcaggg ctgcctgcag ggctggccac tgctcgggga gcagggcagc
      181 cagggtctgc ccccgcagca cagtaggctc ctggcccagg aaggtagcgg cattcaggga
      241 cacttggagc acctctccgg agtggccgtc agctgtgagc agagccccgt gtggctggat
      301 ggacccagga atgtggatag gctctctctc gcagttctcg gttgtaatct cagggccgcc
      361 caggtacaga ggtggaaaga atggcagagg gtcccgagct ccggaggacc caccacctcc
      421 agagccaccg ccaccatcga tgcgttcgcc aactaatttc tttaatctgg caacctcatt
      481 ttccaagtga taatttttcg aaagcaattc ttcaaccttg tcttcaagtt gtttcattct
      541 ttgcaacttt ctcgcacgag aacgcctggc ggcttcagtg ttcattctag aatccggggt
      601 tttttctcct tgacgttaaa gtatagaggt atattaacaa ttttttgttg atacttttat
      661 tacatttgaa taagaagtaa tacaaaccga aaatgttgaa agtattagtt aaagtggtta
      721 tgcagttttt gcatttatat atctgttaat agatcaaaaa tcatcgcttc gctgattaat
      781 taccccagaa ataaggctaa aaaactaatc gcattatcat cctatggttg ttaatttgat
      841 tcgttcattt gaaggtttgt ggggccaggt tactgcccaa tttttcctct tcataaccat
      901 aaaagctagt attgtagaat ctttattgtt cggagcagtg cggcgcgagg cacatctgcg
      961 tttcaggaac gcgaaccggt gaagacgaga cgcacggaag gagagtcttc cttcgaggct
     1021 gtcacccgct cggcggcttc taatccgtac tagagctcca gcttttgttc ccttta
//
