LOCUS       sequence-92153-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of NPC1-CFP sterol sensing domain mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Ko DC, Gordon MD, Jin JY, Scott MP
  TITLE     Dynamic movements of organelles containing Niemann-Pick C1 protein: 
            NPC1 involvement in late endocytic events.
  JOURNAL   Mol Biol Cell. 2001 Mar;12(3):601-14.
  PUBMED    11251074
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2001-03"; volume: "12(3)"; pages: "601-14"
COMMENT     Sequence Label: NPC1-CFP P692S
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    142..263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(179..198)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     233..252
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(270..725)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(407..428)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     619..638
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt
       61 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
      121 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
      181 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
      241 tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt
      301 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
      361 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
      421 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
      481 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
      541 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
      601 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
      661 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
      721 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      781 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      841 ttgaaa
//
