LOCUS       sequence-92164-e        6796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MethyloBrick expression vector, tuf promoter; TcR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Schada von Borzyskowski L, Remus-Emsermann M, Weishaupt R, Vorholt 
            JA, Erb TJ
  TITLE     A Set of Versatile Brick Vectors and Promoters for the Assembly, 
            Expression, and Integration of Synthetic Operons in Methylobacterium
            extorquens AM1 and Other Alphaproteobacteria.
  JOURNAL   ACS Synth Biol. 2014 Aug 8.
  PUBMED    25105793
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Aug 8."
COMMENT     Sequence Label: pTE105
FEATURES             Location/Qualifiers
     source          1..6796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(34..51)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(34..50)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(43..65)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(259..278)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     378..400
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(438..456)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1242..2441
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(2672..3820)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(4092..4463)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            4496..4605
                     /note="incP origin of transfer"
     rep_origin      4758..5389
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      5852..6440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6341..6360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagatcttga ctagtcctgc aggtacctaa ttcactggcc gtcgttttac aacgtcgtga
       61 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
      121 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      181 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
      241 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
      301 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
      361 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
      421 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      481 aatggtttct tagcaccctt tctcggtcct tcaacgttcc tgacaacgag cctccttttc
      541 gccaatccat cgacaatcac cgcgagtccc tgctcgaacg ctgcgtccgg accggcttcg
      601 tcgaaggcgt ctatcgcggc ccgcaacagc ggcgagagcg gagcctgttc aacggtgccg
      661 ccgcgctcgc cggcatcgct gtcgccggcc tgctcctcaa gcacggcccc aacagtgaag
      721 tagctgattg tcatcagcgc attgacggcg tccccggccg aaaaacccgc ctcgcagagg
      781 aagcgaagct gcgcgtcggc cgtttccatc tgcggtgcgc ccggtcgcgt gccggcatgg
      841 atgcgcgcgc catcgcggta ggcgagcagc gcctgcctga agctgcgggc attcccgatc
      901 agaaatgagc gccagtcgtc gtcggctctc ggcaccgaat gcgtatgatt ctccgccagc
      961 atggcttcgg ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc
     1021 ggctgctgaa cccccaaccg ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc
     1081 tcgttcaaca ggtccagggc ggcacggatc actgtattcg gctgcaactt tgtcatgatt
     1141 gacactttat cactgataaa cataatatgt ccaccaactt atcagtgata aagaatccgc
     1201 gcgttcaatc ggaccagcgg aggctggtcc ggaggccaga cgtgaaaccc aacatacccc
     1261 tgatcgtaat tctgagcact gtcgcgctcg acgctgtcgg catcggcctg attatgccgg
     1321 tgctgccggg cctcctgcgc gatctggttc actcgaacga cgtcaccgcc cactatggca
     1381 ttctgctggc gctgtatgcg ttggtgcaat ttgcctgcgc acctgtgctg ggcgcgctgt
     1441 cggatcgttt cgggcggcgg ccaatcttgc tcgtctcgct ggccggcgcc actgtcgact
     1501 acgccatcat ggcgacagcg cctttccttt gggttctcta tatcgggcgg atcgtggccg
     1561 gcatcaccgg ggcgactggg gcggtagccg gcgcttatat tgccgatatc actgatggcg
     1621 atgagcgcgc gcggcacttc ggcttcatga gcgcctgttt cgggttcggg atggtcgcgg
     1681 gacctgtgct cggtgggctg atgggcggtt tctcccccca cgctccgttc ttcgccgcgg
     1741 cagccttgaa cggcctcaat ttcctgacgg gctgtttcct tttgccggag tcgcacaaag
     1801 gcgaacgccg gccgttacgc cgggaggctc tcaacccgct cgcttcgttc cggtgggccc
     1861 ggggcatgac cgtcgtcgcc gccctgatgg cggtcttctt catcatgcaa cttgtcggac
     1921 aggtgccggc cgcgctttgg gtcattttcg gcgaggatcg ctttcactgg gacgcgacca
     1981 cgatcggcat ttcgcttgcc gcatttggca ttctgcattc actcgcccag gcaatgatca
     2041 ccggccctgt agccgcccgg ctcggcgaaa ggcgggcact catgctcgga atgattgccg
     2101 acggcacagg ctacatcctg cttgccttcg cgacacgggg atggatggcg ttcccgatca
     2161 tggtcctgct tgcttcgggt ggcatcggaa tgccggcgct gcaagcaatg ttgtccaggc
     2221 aggtggatga ggaacgtcag gggcagctgc aaggctcact ggcggcgctc accagcctga
     2281 cctcgatcgt cggacccctc ctcttcacgg cgatctatgc ggcttctata acaacgtgga
     2341 acgggtgggc atggattgca ggcgctgccc tctacttgct ctgcctgccg gcgctgcgtc
     2401 gcgggctttg gagcggcgca gggcaacgag ccgatcgctg atcgtggaaa cgataggcct
     2461 atgccatgcg ggtcaaggcg acttccggca agctatacgc gccctagaat tgtcaatttt
     2521 aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga
     2581 agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg
     2641 aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat
     2701 tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct
     2761 gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca
     2821 gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg
     2881 gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca
     2941 cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga
     3001 cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc
     3061 ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat
     3121 tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga
     3181 taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc
     3241 gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt
     3301 tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg
     3361 tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct
     3421 gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga
     3481 cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg
     3541 tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct
     3601 ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga
     3661 tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac
     3721 gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca
     3781 gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg
     3841 ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg
     3901 tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct
     3961 tctcgtactt ggtattccga atcttgccct gcacgaatac cagctccgcg aagtcgctct
     4021 tcttgatgga gcgcatgggg acgtgcttgg caatcacgcg caccccccgg ccgttttagc
     4081 ggctaaaaaa gtcatggctc tgccctcggg cggaccacgc ccatcatgac cttgccaagc
     4141 tcgtcctgct tctcttcgat cttcgccagc agggcgagga tcgtggcatc accgaaccgc
     4201 gccgtgcgcg ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg
     4261 ccattgatgc gggccagctc gcggacgtgc tcatagtcca cgacgcccgt gattttgtag
     4321 ccctggccga cggccagcag gtaggcctac aggctcatgc cggccgccgc cgccttttcc
     4381 tcaatcgctc ttcgttcgtc tggaaggcag tacaccttga taggtgggct gcccttcctg
     4441 gttggcttgg tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc
     4501 cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag
     4561 aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg
     4621 atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag
     4681 gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaag
     4741 atccgtcgac cctttccgac gctcaccggg ctggttgccc tcgccgctgg gctggcggcc
     4801 gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc
     4861 cgccggcgtt gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac
     4921 gttgacactt gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga
     4981 tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac
     5041 gcgagtttcc cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac
     5101 ttgaggggcg cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt
     5161 gctgacagat gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc
     5221 agcatttgca agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct
     5281 tttaaaccaa tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg
     5341 cgcacgccga aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct
     5401 cccatccccc caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc
     5461 caagctgacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6481 gctggccttt tgctcacatg gaattctgta catctagatg catgctcgag cggccgccag
     6541 tgtgatggat atctgcagaa ttcgccctta gtgaagacaa aggtaagctt gctcgatctc
     6601 ggcgagcgcc gcggcggcgc cccggaggcg cagcggctct acgagcaggt cggcgaggcc
     6661 gcgtcctgac ccctcattcg gcgcgatccg ccctgccggc aaacctgccg cttgatcatc
     6721 gtcgcacaag cctctaatcg gtccgcgcct ttacgcccgg cttatggccg gtcccgcggt
     6781 aggattgcac gaagct
//
