LOCUS       sequence-92171-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacitracin-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: PliaI
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     97..114
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(138..195)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    196..216
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    426..447
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      450..493
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(497..515)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(743..762)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
       61 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc
      121 tgcataacgc gaagtaatct tttcggtttt aaagaaaaag ggcagggtgg tgacaccttg
      181 cccttttttg ccggactgca gcggccgcta ctagtatcgt tttccttgtc ttcatcttat
      241 acaagttgga agatatgcaa aacgaatcag agattgattt ttatataaga ccggagtcgt
      301 atctcgcacc ggacccattg gcacaaaaaa acgccatgca cgaggcatgg cgttttaaaa
      361 ccctttacag atgaacactt gcttcccttt ctttaattag gtcggacctt tctgctttgg
      421 ccaatctcta gaagcggccg cgaattccag aaatcatcct tagcgaaagc taaggatttt
      481 ttttatctga aattctgcct cgtgatacgc ctatttttat aggttaatgt catgataata
      541 atggtttctt agacgtcagg tggcactcga gttgatcggg cacgtaagag gttccaactt
      601 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
      661 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
      721 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
      781 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
      841 ttatccggcc
//
