LOCUS       sequence-92172-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty vector, integration at lacA, ampr, mlsr.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: pBS2E
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(129..233)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     301..319
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
       61 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      121 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      181 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      241 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
      301 aataggcgta tcacgaggcc ctttcgtctt caagaatgat gggctaatgt gtgtttacga
      361 caattctcac ttcatacttt tccatcgtca ggtcgcctga caatatgtct cctgtcatta
      421 tgtccttcac actctgatca aacgtgacca gctgtttttc ttccgtgaaa ttcatgacaa
      481 aaatataatc attgtcctga tcctgcctcg cttgtacgga gacgcctttt ccgtgccgaa
      541 ccggaaaaac tggagagaga gacaggtctg tgatcagacc ctcatagaaa tcacgctgaa
      601 attgatcctc caaacgcgcg ccgataaaat acgccttgcc ctgctgatac tcatggcttg
      661 tgaccgctgg cgtgcgcgca taaaaatctt cttgatacac cgcttccact gaagctgtct
      721 ttac
//
