LOCUS       sequence-92178-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lux-reporter vector, integration at sacA, ampr, cmr.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: pBS3Clux
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             306..314
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ttttgatgat tcagaccaaa caagaggtta tgatttcaat aaagggcagt ggcgtgactt
       61 tgtattaaaa ggacataaag atactaatcg ccgtattgat tacagttacg aaatcaatcc
      121 cgtgggaacg ccgcaggaat gtattgacat aattcaaaaa gacattgatg ctacaggaat
      181 atcaaatatt tgttgtggat ttgaagctaa tggaacagta gacgaaatta ttgcttccat
      241 gaagctcttc cagtctgatg tcatgccatt tcttaaagaa aaacaacgtt cgctattata
      301 ttagctaagg aggtaaagaa atgaaatttg gattgttctt ccttaacttc atcaattcaa
      361 caactgttca agaacaaagt atagttcgca tgcaggaaat aacggagtat gttgataagt
      421 tgaattttga acagatttta gtgtatgaaa atcatttttc agataatggt gttgtcggcg
      481 ctcctctgac tgtttctggt tttctgctcg gtttaacaga gaaaattaaa attggttcat
      541 taaatcacat cattacaact catcatcctg tccgcatagc ggaggaagct tgcttattgg
      601 atcagttaag tgaagggaga tttattttag ggtttagtga ttgcgaaaaa aaagatgaaa
      661 tgcatttttt taatcgcccg gttgaatatc aacagcaact atttgaagag tgttatgaaa
      721 tcattaacga tgctttaaca acaggctatt gtaatccaga taacgatttt tatagcttcc
      781 ctaaaatatc tgtaaatccc catgcttata cgccaggcgg acctcggaaa tatgtaacag
      841 caaccagtca tcatattgtt gagtgggcgg ccaaaaaagg tattcctctc atctttaagt
      901 gggatgattc taatgatg
//
