LOCUS       sequence-92179-e         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter construct containing a 715 bp promoter region of the Ihh 
            gene in front of a luciferase gene for in vitro DNA transfection 
            assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Wang W, Lian N, Li L, Moss HE, Wang W, Perrien DS, Elefteriou F, 
            Yang X
  TITLE     Atf4 regulates chondrocyte proliferation and differentiation during 
            endochondral ossification by activating Ihh transcription.
  JOURNAL   Development. 2009 Dec;136(24):4143-53. doi: 10.1242/dev.043281. Epub
            2009 Nov 11.
  PUBMED    19906842
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.043281"; journalName: "Development"; date: "2009-12"; 
            volume: "136"; issue: "24"; pages: "4143-53"
COMMENT     Sequence Label: pIhh715bp-Luc
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    18..152
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(42..61)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     96..115
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(577..597)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcgttgggag ctctccggat ccagacatga taagatacat tgatgagttt ggacaaacca
       61 ccactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      121 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
      181 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
      241 gtatggctga ttatgatctc tagtcaaggc actatacatc aaatattcct tattaacccc
      301 tttacaaatt aaaaagctaa aggtacacaa tttttgagca tagttattaa tagcagacac
      361 tctatgcctg tgtggagtaa gaaaaaacag tatgttatga ttataactgt tatgcctact
      421 tataaaggtt acagaatatt tttccataat tttcttgtat agcagtgcag ctttttcctt
      481 tgtggtgtaa atagcaaagc aagcaagagt tctattacta aacacagcat gactcaaaaa
      541 acttagcaat tctgaaggaa agtccttggg gtcttctacc tttctcttct tttttggagg
      601 agtagaatgt tgagagtcag cagtagcctc atcatcacta gatggcattt cttctgagca
      661 aaacaggtt
//
