LOCUS       sequence-92181-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Xylose-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: PxylA
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     97..114
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(138..195)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    196..216
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    482..503
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      506..549
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(553..571)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        639..743
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
       61 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc
      121 tgcataacgc gaagtaatct tttcggtttt aaagaaaaag ggcagggtgg tgacaccttg
      181 cccttttttg ccggactgca gcggccgcta ctagtattcg ataagcttgg gatcccagat
      241 gcattttatt tcatatagta agtacatcac ctattagttt gttgtttaaa caaactaact
      301 tattttcatc ttatataacc tcgtcagtat tttcaatatt ttttttagtt ttttatgaac
      361 acattagata taataaaggg aagattcgct atgtactatg ttgatactta atttaaagat
      421 taaacaaatg gagtggatga agtggatatc gctgatccga aggcagcagt tttttggcct
      481 tctctagaag cggccgcgaa ttccagaaat catccttagc gaaagctaag gatttttttt
      541 atctgaaatt ctgcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      601 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      661 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      721 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      781 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      841 atgctgaaga tc
//
