LOCUS       sequence-92185-e        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  contains multiple cloning sites (MCSs) for cloning and transient 
            expression of single or multiple guide RNA (gRNA) expression 
            cassettes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  TITLE     Sheen lab CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUC119-MCS
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     18..34
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     18..34
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(287..304)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(287..303)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(296..318)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      516..971
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(603..622)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     813..834
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(988..1007)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cggataacaa tttcacacag gaaacagcta tgaccatggg cgcgccaagc ttaggcctgt
       61 cgacgcccgg gcggtaccgc gatcgctcgc gacctgcagg cataaagccg tcagtgtccg
      121 cataaagaac cacccataat acccataata gctgtttgcc atcgctacct taggaccgtt
      181 atagttaatt accctgttat ccctattaat taagagctcg ctaccttaag agaggatatc
      241 ggcgcgcctc tagaatttaa atggatccta cgtactcgag gaattcactg gccgtcgttt
      301 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      361 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      421 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
      481 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
      541 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
      601 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
      661 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
      721 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
      781 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
      841 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta
      901 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
      961 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     1021 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctg
//
