LOCUS       sequence-92186-e         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encoded a yellow fluorescent calcium ion indicator with inverted 
            fluorescence response for expression in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Zhao Y, Abdelfattah AS, Zhao Y, Ruangkittisakul A, Ballanyi K, 
            Campbell RE, Harrison DJ
  TITLE     Microfluidic cell sorter-aided directed evolution of a protein-based
            calcium ion indicator with an inverted fluorescent response.
  JOURNAL   Integr Biol (Camb). 2014 May 19.
  PUBMED    24840546
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Integr Biol
            (Camb). 2014 May 19."
COMMENT     Sequence Label: pTorPE-Y-GECO1
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             71..82
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             237..254
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             258..290
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             345..401
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
ORIGIN
        1 tttatcgcaa ctctctactg tttctccata cccgtttttt gggctaacag gaggaattaa
       61 ccatgggttt aaagaggaga aaggtcatga acaataacga tctctttcag gcatcacgtc
      121 ggcgttttct ggcacaactc ggcagcttaa ccgacgccgg gacgctgggg ccgtcattgt
      181 taacgccgcg acgtgcgact gcggcgcaag cggcgactga cgcttctaga ggttctcatc
      241 atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt
      301 acgacgaaga tgataaggat ctcgccacaa tggccgactc atcacgtcgt aagtggaata
      361 agacaggtca cgcagtcaga gctataggcc ggctgagctc actcgagggt tatagtaccg
      421 agaaaattgt gcctgttcca aagcaaggga tattaaaagg aaaggtcagc atgtacctat
      481 tgctgaagga tggcggtcgt tatcattgcc agtttgatac ggtatataaa gctaaatcag
      541 tgccctccaa gatgccagga cggcatttta tacagcataa gctccttagg gaggaccgct
      601 ctgatgctaa aaacc
//
