LOCUS       sequence-92187-e         483 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encoded a yellow fluorescent calcium ion indicator with inverted 
            fluorescence response for expression in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Zhao Y, Abdelfattah AS, Zhao Y, Ruangkittisakul A, Ballanyi K, 
            Campbell RE, Harrison DJ
  TITLE     Microfluidic cell sorter-aided directed evolution of a protein-based
            calcium ion indicator with an inverted fluorescent response.
  JOURNAL   Integr Biol (Camb). 2014 May 19.
  PUBMED    24840546
REFERENCE   2  (bases 1 to 483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Integr Biol
            (Camb). 2014 May 19."
COMMENT     Sequence Label: pTorPE-Y-GECO1
FEATURES             Location/Qualifiers
     source          1..483
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(194..208)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 gtagtttacc tgaccatccc cgtcggagtc tgctaccctg atcatttcat caaactcttc
       61 atctgttaac ttctctccaa ggtttgtcat cacgtggcga agctctgctg cgccgatgta
      121 gccattgccg tccttatcaa acacacggaa cgcttctcta atttcctctt cactgtccgt
      181 gtcattcatt tttcttgtca tcatcgtcag gaactcaggg aagccgattg tgccgtcacc
      241 gtcggcatct acttcattga tcatgtcccg cagctctgct tctgaggggt tctgccccag
      301 agaccgcagc accgtcccca gctcctcggt tgtcatcgtc ccatccccgt ccttgtcaaa
      361 tagggagaaa gcctctttaa attctgcgat ctgctcttca gtcagttggt cacgcgtgtc
      421 agcttcccaa ttagtggtca ttttcttcat tacggggccg tcggcgggaa aattcacccc
      481 ata
//
