LOCUS       sequence-92188-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter construct containing a 742 bp promoter region of the Ihh 
            gene in front of a luciferase gene for in vitro DNA transfection 
            assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Wang W, Lian N, Li L, Moss HE, Wang W, Perrien DS, Elefteriou F, 
            Yang X
  TITLE     Atf4 regulates chondrocyte proliferation and differentiation during 
            endochondral ossification by activating Ihh transcription.
  JOURNAL   Development. 2009 Dec;136(24):4143-53. doi: 10.1242/dev.043281. Epub
            2009 Nov 11.
  PUBMED    19906842
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.043281"; journalName: "Development"; date: "2009-12"; 
            volume: "136"; issue: "24"; pages: "4143-53"
COMMENT     Sequence Label: pIhh742bp-Luc
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    12..146
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(36..55)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     90..109
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(571..591)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggagctctcc ggatccagac atgataagat acattgatga gtttggacaa accacaacta
       61 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      121 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
      181 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
      241 ctgattatga tctctagtca aggcactata catcaaatat tccttattaa cccctttaca
      301 aattaaaaag ctaaaggtac acaatttttg agcatagtta ttaatagcag acactctatg
      361 cctgtgtgga gtaagaaaaa acagtatgtt atgattataa ctgttatgcc tacttataaa
      421 ggttacagaa tatttttcca taattttctt gtatagcagt gcagcttttt cctttgtggt
      481 gtaaatagca aagcaagcaa gagttctatt actaaacaca gcatgactca aaaaacttag
      541 caattctgaa ggaaagtcct tggggtcttc tacctttctc ttcttttttg gaggagtaga
      601 atgttgagag tcagcagtag cctcatcatc actagatggc atttcttctg agcaaaacag
      661 gttttcctca ttaaaggcat tccaccactg ctcccattca tcagttccat aggttggaat
      721 ct
//
