LOCUS       sequence-92189-e        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  contains multiple cloning sites (MCSs) for cloning and transient 
            expression of single or multiple guide RNA (gRNA) expression 
            cassettes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  TITLE     Sheen lab CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUC119-MCS
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(239..255)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(239..255)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(252..274)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    263..279
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(287..293,294..311,312..317))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    332..353
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(470..487)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(721..740)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgtaggatcc atttaaattc tagaggcgcg ccgatatcct ctcttaaggt agcgagctct
       61 taattaatag ggataacagg gtaattaact ataacggtcc taaggtagcg atggcaaaca
      121 gctattatgg gtattatggg tggttcttta tgcggacact gacggcttta tgcctgcagg
      181 tcgcgagcga tcgcggtacc gcccgggcgt cgacaggcct aagcttggcg cgcccatggt
      241 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      301 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      361 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      421 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      481 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      541 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      601 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      661 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      721 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      781 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      841 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      901 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      961 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agca
//
