LOCUS       sequence-92190-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLlac0-1:RhyB-mCherry, pLtetO-1:RBS (st7) gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 1011
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    270..286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(338..424)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     577..594
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(680..700)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc
       61 atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat cgtacgtttc
      121 tcctctttaa taagcttgtg gataaattga gaacgaaaga tcaaaaaaaa agccagcacc
      181 cggctggcta agtaatactg gaagcaatgt gagcaatgtc gtgctttcag gttctccgcg
      241 agggtcttcc tgatcgcccc gggagtatct tgttatccgc tcacaatgtc aatgttatcc
      301 gctcacattt atggatcccc taggtctagg gcggcggatt tgtcctactc aggagagcgt
      361 tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta
      421 tttgatgcct ggcagttccc tactctcgca tggggagacc ccacactacc atcggcgcta
      481 cggcgtttca cttctgagtt cggcatgggg tcaggtggga ccaccgcgct actgccgcca
      541 ggcaaattct gttttatcag accgcttctg cgttctgatt taatctgtat caggctgaaa
      601 atcttctctc atccgccaaa acagccaagc tagcttttat ttgtagagct catccatgcc
      661 atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc tcttttcgnt
      721 gggatctttc caaagggcag attgtgtgga caggtaat
//
