LOCUS       sequence-92194-e         565 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Truncated form of SpCas9 without C' domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, 
            Ishitani R, Zhang F, Nureki O
  TITLE     Crystal structure of Cas9 in complex with guide RNA and target DNA.
  JOURNAL   Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. 
            Epub 2014 Feb 13.
  PUBMED    24529477
REFERENCE   2  (bases 1 to 565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.02.001"; journalName: "Cell"; date: 
            "2014-02-27- 27"; volume: "156"; issue: "5"; pages: "935-49"
COMMENT     Sequence Label: PX544 SpCas9(del1098-1368)
FEATURES             Location/Qualifiers
     source          1..565
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc
       61 cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc
      121 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac
      181 cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc
      241 agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga
      301 aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc
      361 gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg
      421 gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc
      481 atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc
      541 tctatgatca agagatacga cgagc
//
