LOCUS       sequence-92196-e         378 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encoded a yellow fluorescent calcium ion indicator with inverted 
            fluorescence response for expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Zhao Y, Abdelfattah AS, Zhao Y, Ruangkittisakul A, Ballanyi K, 
            Campbell RE, Harrison DJ
  TITLE     Microfluidic cell sorter-aided directed evolution of a protein-based
            calcium ion indicator with an inverted fluorescent response.
  JOURNAL   Integr Biol (Camb). 2014 May 19.
  PUBMED    24840546
REFERENCE   2  (bases 1 to 378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Integr Biol
            (Camb). 2014 May 19."
COMMENT     Sequence Label: CMV-Y-GECO1
FEATURES             Location/Qualifiers
     source          1..378
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(273..287)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 atgcatgctc gagcggccgc cagtgtgatg gatatctgca gaattcttac ttcgctgtca
       61 tcatttgtac aaactcttcg tagtttacct gaccatcccc gtcggagtct gctaccctga
      121 tcatttcatc aaactcttca tctgttaact tctctccaag gtttgtcatc acgtggcgaa
      181 gctctgctgc gccgatgtag ccattgccgt ccttatcaaa cacacggaac gcttctctaa
      241 tttcctcttc actgtccgtg tcattcattt ttcttgtcat catcgtcagg aactcaggga
      301 agccgattgt gccgtcaccg tcggcatcta cttcattgat catgtcccgc agctctgctt
      361 ctgaggggtt ctgcccca
//
