LOCUS       sequence-92197-e         550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Truncated form of SpCas9 without C' domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, 
            Ishitani R, Zhang F, Nureki O
  TITLE     Crystal structure of Cas9 in complex with guide RNA and target DNA.
  JOURNAL   Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. 
            Epub 2014 Feb 13.
  PUBMED    24529477
REFERENCE   2  (bases 1 to 550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.02.001"; journalName: "Cell"; date: 
            "2014-02-27- 27"; volume: "156"; issue: "5"; pages: "935-49"
COMMENT     Sequence Label: PX544 SpCas9(del1098-1368)
FEATURES             Location/Qualifiers
     source          1..550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggccttatcc agttcgctca ggccgcctct ctcggccttg gtcagattgt cgaactttct
       61 ctgggtaatc agcttggcgt tcagcagctg ccgccagtag ttcttcatct tcttcacgac
      121 ctcttcggag ggcacgttgt cgctcttgcc ccggttcttg tcgcttctgg tcagcacctt
      181 gttgtcgatg gagtcgtcct tcagaaagct ctgaggcacg atatggtcca catcgtagtc
      241 ggacagccgg ttgatgtcca gttcctggtc cacgtacata tcccgcccat tctgcaggta
      301 gtacaggtac agcttctcgt tctgcagctg ggtgttttcc acggggtgtt ctttcaggat
      361 ctggctgccc agctctttga tgccctcttc gatccgcttc attctctcgc ggctgttctt
      421 ctgtcccttc tgggtggtct ggttctctct ggccatttcg atcacgatgt tctcgggctt
      481 gtgccggccc atcactttca cgagctcgtc caccaccttc actgtctgca ggatgccctt
      541 cttaatggcg
//
