LOCUS       sequence-92198-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Truncated form of SpCas9 without C' domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, 
            Ishitani R, Zhang F, Nureki O
  TITLE     Crystal structure of Cas9 in complex with guide RNA and target DNA.
  JOURNAL   Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. 
            Epub 2014 Feb 13.
  PUBMED    24529477
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.02.001"; journalName: "Cell"; date: 
            "2014-02-27- 27"; volume: "156"; issue: "5"; pages: "935-49"
COMMENT     Sequence Label: PX544 SpCas9(del1098-1368)
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..58)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ggaattctta ctttttcttt tttgcctggc cggccttttt cgtggccgcc ggccttttgg
       61 tctttttcac gatattcact tggggcatgc tcagcacttt ccgcacggtg gcaaaatccc
      121 ggcccttatc ccacacgatc tccccggttt cgccgtttgt ctcgatcaga ggccgcttcc
      181 ggatctcgcc gttggccagg gtaatctcgg tcttgaaaaa gttcatgatg ttgctgtaga
      241 agaagtactt ggcggtagcc ttgccgattt cctgctcgct cttggcgatc atcttccgca
      301 cgtcgtacac cttgtagtcg ccgtacacga actcgctttc cagcttaggg tactttttga
      361 tcagggcggt tcccacgacg gcgttcaggt aggcgtcgtg ggcgtggtgg tagttgttga
      421 tctcgcgcac tttgtaaaac tggaaatcct tccggaaatc ggacaccagc ttggacttca
      481 gggtgatcac tttcacttcc cggatcagct tgtcattctc gtcgtactta gtgttcatcc
      541 gggagtccag gatctgtgcc acgtgctttg tgatctgccg ggtttccacc agctgtctct
      601 tgatgaagcc ggccttatcc agttcgctca ggccgcctct ctcggccttg gtcagattgt
      661 cgaactttct ctgggtaatc agcttggcgt tcagcagctg ccgccagtag ttcttcatct
      721 tcttcacgac ctcttcggag ggcacgttgt cg
//
