LOCUS       sequence-92202-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used for anti-mouse Smoothened antibody production (for affinity 
            purification).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Rohatgi R, Milenkovic L, Scott MP
  TITLE     Patched1 regulates hedgehog signaling at the primary cilium.
  JOURNAL   Science. 2007 Jul 20;317(5836):372-6.
  PUBMED    17641202
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2007-07-20"; volume: "317(5836)"; pages: "372-6"
COMMENT     Sequence Label: pET28 mSmo-Cterm
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..81
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 gagaatcttt attttcaggg ccggccaatg aaggccaccc tgctcatctg gaggcgcacc
      121 tggtgcaggt tgactgggca cagtgatgat gagcccaaga gaatcaagaa gagcaagatg
      181 atcgccaagg ccttctctaa gcggcgtgag ctgctgcaga acccgggcca ggagctctcc
      241 ttcagcatgc acactgtctc ccatgatgga cctgttgcgg gtttggcttt tgacctcaat
      301 gaaccctcag ctgacgtctc ctctgcctgg gctcagcatg tcaccaagat ggtggctcgg
      361 agaggagcca tattgcccca ggatgtgtcc gttacccctg tggcaactcc agtgccacca
      421 gaagaacaag ccaacatgtg gctggttgag gcagagatct ccccagagtt agagaagcgt
      481 ttgggccgga agaaaaagcg gaggaagagg aagaaggagg tgtgcccctt ggggccagcc
      541 cctgagcttc accactctgc ccctgttcct gccaccagtg cagttcctcg gctgcctcag
      601 ctgcctcggc agaagtgcct ggtagctgca aacgcctggg gaacagggga gtcctgccga
      661 cagggagcct ggactctagt ctccaacccc ttctgcccag agcctagtcc ccatcaagat
      721 ccatttctcc ctggtgcctc agcccc
//
