LOCUS       sequence-92204-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-tagged mouse TRAF4 K190R mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Wang X, Jin C, Tang Y, Tang LY, Zhang YE
  TITLE     Ubiquitination of tumor necrosis factor receptor-associated factor 4
            (TRAF4) by Smad ubiquitination regulatory factor 1 (Smurf1) 
            regulates motility of breast epithelial and cancer cells.
  JOURNAL   J Biol Chem. 2013 Jul 26;288(30):21784-92. doi: 
            10.1074/jbc.M113.472704. Epub 2013 Jun 11.
  PUBMED    23760265
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.472704"; journalName: "J Biol Chem"; date: 
            "2013-07-26- 26"; volume: "288"; issue: "30"; pages: "21784-92"
COMMENT     Sequence Label: pRK-FLAG-TRAF4(K190R)
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..69
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cactcccagg tccaactgca cctcggttct atcgccgcca ccatggacta caaggacgac
       61 gatgacaaga tcgatgaatt cggatccatg cccggcttcg actacaagtt cctggagaag
      121 cccaagcggc ggctgctgtg cccactgtgc gggaagccca tgcgcgagcc tgtgcaggtg
      181 tctacttgtg gccaccgctt ctgcgacacc tgcctgcagg agtttctcag tgaaggagtc
      241 ttcaaatgcc ctgaggacca acttcctctg gactatgcca agatctaccc agacccagag
      301 ctggaggtcc aggtgttagg cttggctatc cgctgcatcc acagtgagga gggctgccgc
      361 tggagcgggc cacttcgtca tctacagggc cacttgaaca cttgcagctt caatgtagtc
      421 ccctgcccta atcgctgccc agccaagctg agccggcgtg acctgcctgc ccacctgcag
      481 cacgactgcc ctaagcgccg cctcaagtgt gaattctgtg gctgtgactt cagtggggag
      541 gcctacgaga gtcacgaggg cgtgtgcccc caagagagtg tgtactgtga gaacaagtgt
      601 ggggcccgca tgatgaggcg actgttggcc cagcatgcca cgtctgagtg cccccggcgc
      661 acccagccct gtgcctactg taccaaggaa tttgtttatg acaccatcca gagccaccag
      721 taccagtgcc cacgcctgcc tgttccctgc cccaaccaat gtggagtggg cactgtggcc
      781 cgggaagacc tgcccaccca tctgaaggat agctgcagca ctgcctttgt tctctgccct
      841 ttcaaagagt ctggctgcaa gcacaggtgc cctaagctgg caatgggacg tcacgtggag
      901 gagagcgtaa agccacatct ggctatgatg tgtgccctgg tg
//
