LOCUS       sequence-92211-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 6, small linker insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_6_Linker
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        42..60
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     42..59
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          157..729
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(826..845)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agggaaccgc cattctgcct ggggacgtcg gagcaagctt gatttaggtg acactataga
       61 ggtctcatca ggcggatccg gcggcggtac cagtttgaga ccctcatcct cccgggtaac
      121 taagtaagga tctcgaccga tcctgagaac ttcagggtga gtttggggac ccttgattgt
      181 tctttctttt tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg
      241 ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc
      301 tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta
      361 actttttcgt taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat
      421 ttgtcagatt gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta
      481 tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata
      541 tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacacc
      601 ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg
      661 ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttctct
      721 ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt tggcaaagaa
      781 ttaattcctc gacggatcgt agatccagac atgataagat acattgatga gtttggacaa
      841 accacaacta g
//
