LOCUS       sequence-92218-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse genomic ROSA26 targeting vector, expresses EGFP, cre/lox 
            conditional U6 promoter pgk-puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Brown JR, Zetsche B, Jackson-Grusby L
  TITLE     RUSH and CRUSH: a rapid and conditional RNA interference method in 
            mice.
  JOURNAL   Genesis. 2014 Jan;52(1):39-48. doi: 10.1002/dvg.22718. Epub 2013 Oct
            26.
  PUBMED    24166816
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg.22718";
            journalName: "Genesis"; date: "2014-01"; volume: "52"; issue: "1"; 
            pages: "39-48"
COMMENT     Sequence Label: pCRUSH
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    47..271
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    323..547
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctgtaca agtaaagcgg ccctagagct cgctgatcag cctcgactgt gccttctagt
       61 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
      121 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
      181 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
      241 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
      301 tcgactcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
      361 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
      421 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
      481 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
      541 tctatggctt ctgaggcgga aagaaccagc tggggctcga tcctctagag atccgacgcg
      601 ccatctctag gcccgcgccg gccccctcgc acagacttgt gggagaagct cggctactcc
      661 cctgccccgg ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat
      721 aa
//
