LOCUS       sequence-92219-e         532 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Truncated form of SpCas9 without C' domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, 
            Ishitani R, Zhang F, Nureki O
  TITLE     Crystal structure of Cas9 in complex with guide RNA and target DNA.
  JOURNAL   Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. 
            Epub 2014 Feb 13.
  PUBMED    24529477
REFERENCE   2  (bases 1 to 532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.02.001"; journalName: "Cell"; date: 
            "2014-02-27- 27"; volume: "156"; issue: "5"; pages: "935-49"
COMMENT     Sequence Label: PX544 SpCas9(del1098-1368)
FEATURES             Location/Qualifiers
     source          1..532
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             62..127
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             134..154
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggaccg gtgccaccat
       61 ggactataag gaccacgacg gagactacaa ggatcatgat attgattaca aagacgatga
      121 cgataagatg gccccaaaga agaagcggaa ggtcggtatc cacggagtcc cagcagccga
      181 caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg ccgtgatcac
      241 cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag
      301 catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac
      361 ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct
      421 gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga
      481 agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct tc
//
