LOCUS       sequence-92222-e         536 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Truncated form of SpCas9 without REC2 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, 
            Ishitani R, Zhang F, Nureki O
  TITLE     Crystal structure of Cas9 in complex with guide RNA and target DNA.
  JOURNAL   Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. 
            Epub 2014 Feb 13.
  PUBMED    24529477
REFERENCE   2  (bases 1 to 536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.02.001"; journalName: "Cell"; date: 
            "2014-02-27- 27"; volume: "156"; issue: "5"; pages: "935-49"
COMMENT     Sequence Label: PX543 SpCas9(del175-307)
FEATURES             Location/Qualifiers
     source          1..536
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttcgctcagg ccgcctctct cggccttggt cagattgtcg aactttctct gggtaatcag
       61 cttggcgttc agcagctgcc gccagtagtt cttcatcttc ttcacgacct cttcggaggg
      121 cacgttgtcg ctcttgcccc ggttcttgtc gcttctggtc agcaccttgt tgtcgatgga
      181 gtcgtccttc agaaagctct gaggcacgat atggtccaca tcgtagtcgg acagccggtt
      241 gatgtccagt tcctggtcca cgtacatatc ccgcccattc tgcaggtagt acaggtacag
      301 cttctcgttc tgcagctggg tgttttccac ggggtgttct ttcaggatct ggctgcccag
      361 ctctttgatg ccctcttcga tccgcttcat tctctcgcgg ctgttcttct gtcccttctg
      421 ggtggtctgg ttctctctgg ccatttcgat cacgatgttc tcgggcttgt gccggcccat
      481 cactttcacg agctcgtcca ccaccttcac tgtctgcagg atgcccttct taatgg
//
