LOCUS       sequence-92223-e         552 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Truncated form of SpCas9 without REC2 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, 
            Ishitani R, Zhang F, Nureki O
  TITLE     Crystal structure of Cas9 in complex with guide RNA and target DNA.
  JOURNAL   Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. 
            Epub 2014 Feb 13.
  PUBMED    24529477
REFERENCE   2  (bases 1 to 552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.02.001"; journalName: "Cell"; date: 
            "2014-02-27- 27"; volume: "156"; issue: "5"; pages: "935-49"
COMMENT     Sequence Label: PX543 SpCas9(del175-307)
FEATURES             Location/Qualifiers
     source          1..552
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc
       61 cacatgatca agttccgggg ccacttcctg atcgagggcg acctggtgaa caccgagatc
      121 accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca ccaggacctg
      181 accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga gattttcttc
      241 gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca ggaagagttc
      301 tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact gctcgtgaag
      361 ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag catcccccac
      421 cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt ttacccattc
      481 ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc ctactacgtg
      541 ggccctctgg cc
//
