LOCUS       sequence-92224-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Truncated form of SpCas9 without REC2 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, 
            Ishitani R, Zhang F, Nureki O
  TITLE     Crystal structure of Cas9 in complex with guide RNA and target DNA.
  JOURNAL   Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. 
            Epub 2014 Feb 13.
  PUBMED    24529477
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.02.001"; journalName: "Cell"; date: 
            "2014-02-27- 27"; volume: "156"; issue: "5"; pages: "935-49"
COMMENT     Sequence Label: PX543 SpCas9(del175-307)
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..58)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ggaattctta ctttttcttt tttgcctggc cggccttttt cgtggccgcc ggccttttgt
       61 cgcctcccag ctgagacagg tcgatccgtg tctcgtacag gccggtgatg ctctggtgga
      121 tcagggtggc gtccagcacc tctttggtgc tggtgtacct cttccggtcg atggtggtgt
      181 caaagtactt gaaggcggca ggggctccca gattggtcag ggtaaacagg tggatgatat
      241 tctcggcctg ctctctgatg ggcttatccc ggtgcttgtt gtaggcggac agcactttgt
      301 ccagattagc gtcggccagg atcactctct tggagaactc gctgatctgc tcgatgatct
      361 cgtccaggta gtgcttgtgc tgttccacaa acagctgttt ctgctcatta tcctcggggg
      421 agcccttcag cttctcatag tggctggcca ggtacaggaa gttcacatat ttggagggca
      481 gggccagttc gtttcccttc tgcagttcgc cggcagaggc cagcattctc ttccggccgt
      541 tttccagctc gaacagggag tacttaggca gcttgatgat caggtccttt ttcacttctt
      601 tgtagccctt ggcttccaga aagtcgatgg gattcttctc gaagctgctt ctttccatga
      661 tggtgatccc cagcagctct ttcacactct tcagtttctt ggacttgccc ttttccactt
      721 tggccaccac cagcaca
//
