LOCUS       sequence-92233-e        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  photo-activatable mitochondrial marker for expression in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Pham AH, McCaffery JM, Chan DC
  TITLE     Mouse lines with photo-activatable mitochondria to study 
            mitochondrial dynamics.
  JOURNAL   Genesis. 2012 Nov;50(11):833-43. doi: 10.1002/dvg.22050. Epub 2012 
            Aug 11.
  PUBMED    22821887
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg.22050";
            journalName: "Genesis"; date: "2012-11"; volume: "50"; issue: "11"; 
            pages: "833-43"
COMMENT     Sequence Label: mito-dendra2
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    127..248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(138..157)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     192..211
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(398..1084)
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="NTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTAN
                     LTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKGI
                     CTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNINM
                     ALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARYS
                     PLPSQVW"
ORIGIN
        1 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
       61 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
      121 acgccttaag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
      181 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      241 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      301 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt
      361 cgcggccgct ttacttgtac acgccgctgt cgccggacca cacctggctg ggcagggggc
      421 tgtagcgggc cacggcgtgc tcgtacagct tcaccttgtt gtagtcgctg tcgttgccca
      481 ggatctcgat gcggtggtcc acgaagtggg cgtcgggcag ctgcaccacc ttcttggcct
      541 tgtaggtggt cttgaagtcg cacaggtagt ggccgccgcc ctccagcagc agggccatgt
      601 tgatgttgcc caccagcagg ccgtcgcgca cgtgcagctt ctcggtgctg ggctcccact
      661 tcagggtctt cttctgcatc acggggccgt tgggggggaa gttggtgccc ttgaagcgca
      721 cgttctggaa gaagcagtcg ccctccaggc tgatgtcgct gcggatggtg cagatgccct
      781 tgtcctcgaa ggtcatggtg cgctcccagc tgtagccctc ggggaagctc tgcttgaagt
      841 agtcggggat gtcctcgggg tacttggtga acacccggtt gccgtagtgc acggcggtgg
      901 tcaggatgtc gtagctgaag ggcagggggg cgccctcctt cacggtcagg ttggcggtct
      961 gggtgccctc gtagggcttg cccttgccct cgccctcgat cacgaaggcg tggccgttca
     1021 cgttgccctc catgtgcacc ttcacgcgca tgtcctcctt gatcaggtta attcccgggg
     1081 tgttcggatc cccca
//
