LOCUS       sequence-92235-e        1114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  photo-activatable mitochondrial marker for expression in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Pham AH, McCaffery JM, Chan DC
  TITLE     Mouse lines with photo-activatable mitochondria to study 
            mitochondrial dynamics.
  JOURNAL   Genesis. 2012 Nov;50(11):833-43. doi: 10.1002/dvg.22050. Epub 2012 
            Aug 11.
  PUBMED    22821887
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg.22050";
            journalName: "Genesis"; date: "2012-11"; volume: "50"; issue: "11"; 
            pages: "833-43"
COMMENT     Sequence Label: mito-dendra2
FEATURES             Location/Qualifiers
     source          1..1114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..119
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             141..827
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="NTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTAN
                     LTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKGI
                     CTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNINM
                     ALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARYS
                     PLPSQVW"
     polyA_signal    977..1098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1014..1033)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1068..1087
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcatgtcc gtcctgacgc
       61 cgctgctgct gcggggcttg acaggctcgg cccggcggct cccagtgccg cgcgccaaga
      121 tccattcgtt gggggatccg aacaccccgg gaattaacct gatcaaggag gacatgcgcg
      181 tgaaggtgca catggagggc aacgtgaacg gccacgcctt cgtgatcgag ggcgagggca
      241 agggcaagcc ctacgagggc acccagaccg ccaacctgac cgtgaaggag ggcgcccccc
      301 tgcccttcag ctacgacatc ctgaccaccg ccgtgcacta cggcaaccgg gtgttcacca
      361 agtaccccga ggacatcccc gactacttca agcagagctt ccccgagggc tacagctggg
      421 agcgcaccat gaccttcgag gacaagggca tctgcaccat ccgcagcgac atcagcctgg
      481 agggcgactg cttcttccag aacgtgcgct tcaagggcac caacttcccc cccaacggcc
      541 ccgtgatgca gaagaagacc ctgaagtggg agcccagcac cgagaagctg cacgtgcgcg
      601 acggcctgct ggtgggcaac atcaacatgg ccctgctgct ggagggcggc ggccactacc
      661 tgtgcgactt caagaccacc tacaaggcca agaaggtggt gcagctgccc gacgcccact
      721 tcgtggacca ccgcatcgag atcctgggca acgacagcga ctacaacaag gtgaagctgt
      781 acgagcacgc cgtggcccgc tacagccccc tgcccagcca ggtgtggtcc ggcgacagcg
      841 gcgtgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga
      901 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
      961 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1021 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1081 actcatcaat gtatcttaag gcgtaaattg taag
//
