LOCUS       sequence-92240-e        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  replace TgUPRT with DHFR by homologous recombination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Shen B, Brown KM, Lee TD, Sibley LD
  TITLE     Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii 
            Using CRISPR/CAS9.
  JOURNAL   MBio. 2014 May 13;5(3). pii: e01114-14. doi: 10.1128/mBio.01114-14.
  PUBMED    24825012
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2014-05-13"; volume: "5(3). pii: e01114-14. doi"; pages: " 
            10.1128/mBio.01114-14"
COMMENT     Sequence Label: pUPRT::DHFR-D
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1012..1036
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgtataataa agttgtcgac gtcaactgta cgacatccga gtgaaaacag tctctgcccg
       61 ttggtcccgt agttcaaata actcgataaa ttagcagaca gtttttcggc aacctcagaa
      121 gagagaagtt gtgtgctaga ggttccaccc tcgtgggagc gaaaaaacag aatgctggtg
      181 gcgcacttca tcagagcaga cttggtactg ccatggtctg ggcaatgaca ctgttcaatc
      241 cccaccgtgg aaaaaaaaac acgccaactt ccctacacga actggcaagc gtgacatcga
      301 taccgactgt atgtggaaac gcgtttgctt cacccaggac gtggtcgcac attacgggtt
      361 ttcgagggtt cgacatcgca tatgcattgc ttgtccatcc cacaccgtta aaattccgtt
      421 agagaatgta acgtggacca ttcttcacat tgcttcaaca acatctagat atgaggttgt
      481 aaatgtttcc gagcagcgcg aacaaaaagc aaagaacggc ggaaacacac aaggtaatcg
      541 tgaagaacac ttcttgggca caagtaaacg acttaaggtg aagaggagca attaagctgc
      601 gcccttcctg gcactaccac ttcgcttccc tgtctcgttc tggacagcag aatctcgaac
      661 aaaggaaagg gaaataaaac tcgagatgga cgaggaacgg gatgcacgcc gcctcgtaaa
      721 cattcccgtt acaggtgtac gggactctgt gcctgatacg aagaaatcgt ccggtccatg
      781 actaacaaca gtcgtgacag gagcgcgacc aaagatatac cccgtggcac aaaaaagcgc
      841 aactgcttgt cgcgcaccag gcagcctcac aactaaaact ttttttctgt gttcctgccg
      901 atggtgaggg acgtgggagc gagacaaaaa taaaaatggt gtttatcctc ttgaggcgtg
      961 ctttttccag tccgcgattc cgtcagcggt ctgtcaaaaa aactagagac gaccactttg
     1021 tacaagaaag ctgggtcgga attcatcctg caagtgcata gaaggaaagt tgtctgctgt
     1081 c
//
