LOCUS       sequence-92242-e        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  replace TgUPRT with DHFR by homologous recombination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Shen B, Brown KM, Lee TD, Sibley LD
  TITLE     Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii 
            Using CRISPR/CAS9.
  JOURNAL   MBio. 2014 May 13;5(3). pii: e01114-14. doi: 10.1128/mBio.01114-14.
  PUBMED    24825012
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2014-05-13"; volume: "5(3). pii: e01114-14. doi"; pages: " 
            10.1128/mBio.01114-14"
COMMENT     Sequence Label: pUPRT::DHFR-D
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    998..1022
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actttgtata gaaaagttgt cccgctgtgc ctagtatcga aagctgtaga gttgccaccc
       61 tgatcttctc ccttccaact ctccgcttcg acacaccccc caacgacttg ttgtgaagct
      121 ctggcccccc tgtttcggtt gtgacaggct ctccaggtgc tcttgcactc tccagtccag
      181 ctggtgtcat agaaccgtta tctctagagt gccccgaaag agccccctcc ttttattcgt
      241 tgtttcccct gggtacgagc tatcttgtgg ctttctttgc ttggggatga cccttgtcgt
      301 gaccttctat gcgttctcga tgagaaaccg tcgagtcttc gttgcatgca ggggtttctg
      361 cgcgaagata ctctcccaca gagccacaga aagtggtagc cgctgttttc caacgcagcg
      421 tacgtaccta gagatccttc caggaaacgc tgagcctccg ctgtgactga ttttctgcac
      481 gttggcacgt ggatatcgcg atcccctgtc ccctccactg catgcagtgt ggcggtaaga
      541 cgagggccat atcgatccca tggcagctgc tgtgtggggt gccgccatac gtaggggtac
      601 gatttctggt cctgcccaac ttcccgcctg gttgattcga gcaacttaca aggacaaggc
      661 aattaaccat cttgctattt ttcctgtttt cgtcgtcatc ggctgtccta gctctcacgg
      721 ctacccatcg ttttcccaag cgctgtcaag tgatgccatt ggttacactg gacaaaggcc
      781 ctgcaaatac gcgtgcctcc atcatcgatc cccctttatc tactttctcg ctttccgtta
      841 tcccttctgt ctgctttttc ctttctcggc tccacctcat tcccctcgtt ccttgtagcg
      901 gccccggctt ctacgttccg cctgcgattc ccgtattggt cacgcatcca caaggcgtat
      961 tccttttttc gtcggacctg tccacagggc ttctaaaaca agtttgtaca aaaaagcagg
     1021 cttcaagctt cgccaggctg taaatcccgt gagtcgtcct c
//
