LOCUS       sequence-92253-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 
            528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: mCherry-miniSOG-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(143..850)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(418..437)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     687..705
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccgctacag cttggatccg gacttgtaca gctcgtccat gccgccggtg gagtggcggc
      181 cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
      241 acttgatgtt gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg
      301 tcttgacctc agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc
      361 ccttcagggc gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggtct
      421 tcttctgcat tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga
      481 tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga
      541 agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga
      601 tgtcggcggg gtgcttcacg taggccttgg agccgtacat gaactgaggg gacaggatgt
      661 cccaggcgaa gggcaggggg ccacccttgg tcaccttcag cttggcggtc tgggtgccct
      721 cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct
      781 ccatgtgcac cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct
      841 tgctcaccat gccgccgccg tccagctgca cgccgatgaa gtactgcagc tcgc
//
