LOCUS       sequence-9226-ex        5647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5647)
  TITLE     Forth generation Seydoux lab vectors
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCM1.52
FEATURES             Location/Qualifiers
     source          1..5647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    3692..3816
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3888..3907)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3889..3907)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3912..3928)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3912..3928)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4030..4049)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4041..4850
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4108..4127)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4997..5585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5486..5505
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgtg ccaaaggctt ggtttttata agaattgttt
      721 caataagcat aacattctta atgttttatg tatctctacg gtaaaaaaga tacaaattga
      781 aatctaaaat agtgacaaat ctgaaacgaa cttatatcga aaataggctc tctgtttttg
      841 ttttgtccat tatattttta aaatatgaat gagtatggaa aactccaaaa aaaaattcaa
      901 aatgttcctg atacaccact caatttacac atttcattca tgcaaatgcg cctattttcc
      961 aatattttag agaactattc tttgcatatt taaaattttg atacttttga aactataaaa
     1021 tttcattcta gactcttttt ctctcaacgt gtgaaaattt gaatgagaag agaaaattta
     1081 ttttcggatt ttgtttattt ttctcattta tttttagtct caaattgctt cacaccgtaa
     1141 atagtttgaa gatttttttt gttgaagaaa ctaaaaattt agattttttc gttcattctt
     1201 tcgtattttt caatgaaatc ggagattctg attctgattt ctatttcatg aaagagagaa
     1261 aaaatcaaag aaaaaacggg gatgatatcc atttcgacca ccacatagat tccatttaat
     1321 cttttagttt aactttctgt ttttttagaa aaaatttcta tacgaaaaaa aaaggctgcc
     1381 tcatgcctgc ctgtataccg gcaagtttcc gcattttggc ataaactcac aaggtgcttg
     1441 gtttttgaaa aagaataata cacaattttt tatttgtaaa aattaccaaa agttcaacaa
     1501 taatatatcg tgaagtttgg caggcagtcg taggcacctt gtaggcgaac ctatccagaa
     1561 tgtaaatttt tcgggaataa tattttttat actcagaata aaaaataatt tcacaccagt
     1621 tcataatttt tctcacgaca aattctctat gaaattctgc taaacagcct ccgcgcgaac
     1681 tttccaaaat ttcgaaatcg tgaatctaat aaccaaaaaa ccaatttatt tgtcattctg
     1741 atttctgcaa aaaacacaaa agtgggggag gccactccat tttggagcta ataatagtgt
     1801 gctccaaaaa aaactttttc atctatctac gtcaaaactt ttcggccgtc gtcctatttt
     1861 taggctcgtc gtttcctctc cattttttcc ttttttcggc ggcgtcacaa cgaattaatg
     1921 aatttattgg agcgaaaacg ttgctccgac ggggcggaga agggcacact agaatttatg
     1981 aatggtgtgc tcttcgaata gaaaaagagc acctggaggc agcaattgct tcagattttt
     2041 taaaatctag tttcacacgt ataatcttac gatcctcttc gatgtgcaat gtaagcgacc
     2101 gacattttat gggggttttc gaaatttcaa aaacaacccg tgaaacgtcg gatgcattat
     2161 atttcctgat tctatgcaat aaacagttta tagatcaaaa atcagatccc aatattgcta
     2221 gaatctccca aatgaaaatg aacttgaccg tgggaaaatt tttaaaatat ctagccaagg
     2281 atttgtagac aaatcgtgcg aaaatcggtc caaaaaaact cacattcttc gagtgttctc
     2341 ttctcgagat ccacctccgc gcctatgatt tcttccttcc attctttttt ctctatttct
     2401 ccaaccaaaa atagtaagga aaaaatagtg aaaaaatgaa ggtcacttcc ttttttctgt
     2461 ctctctcttt cccatatcaa tcacctgatt tgcgatcatc gccatgtctt ttctcgccct
     2521 gttattttgc tgctaagttt gttttttttt tcagtttttg ttgttctcct acaccgcttt
     2581 ccggtgtttt cccattggtt agtgattaat gattacaagt tggaaatgcc aacattttga
     2641 tctgttccta ttggttttag aattctcgag gtttaaggtt ttcatttgaa atggaaagta
     2701 ttctttaact taagatcata cattttttcc agtattcaac tcgtagagta tataatctag
     2761 ttttacatat tcccacattt ccatagtttc accattatct ccgacggaag ctgtacatgt
     2821 tttcctaaga ttttattata aaactgatta tcatgaaaac ttatcattac tgataagaga
     2881 cgcatatttc tcgtgggcgc cagcgcccac ggatacctag gaactcaaaa gtgaaatatt
     2941 tctttttgat atgagcgcat gatgacgagc caaggattat ccctctctca cagcttcttt
     3001 tcttcgtccc gtagatctgt ttgagccatt cttagcgagg attgtcgatt tagcagtcga
     3061 cgcgcatgaa ttttgctcaa aaacactgat atttattgag aattttcatg ctccattttc
     3121 aaaaaaaaaa gtctctgaga tatttgcagg tattttattc ggataatttt tattttgtac
     3181 tggtgtgtct tgttgaagga tgccgcagca cattcaacta tctaaaaaaa caggcgaatt
     3241 ttaattttat taaatttcag ttcttcaatt aaatagtttt gattgaatta tgaatatttt
     3301 tatatatcta gatttgaaaa taaatttcaa acccataaaa attaaatatt cacatgtcgt
     3361 tgcgccatgc gcctttaaaa aatgacagta cttccataat ttcaaacgcg ggaaaaaatt
     3421 aatttgattt tctatttttt cgctcatcac atcgatttcc caaattttct cgaatttttt
     3481 ttctcagaag agtttttgat tttttctaaa ttatcccgct gacgctgccc actcacagaa
     3541 tattttatta tcgatgaaat gactcctacg tattttcgtg tctccccgct gtttcaccag
     3601 tgttctatta tttccaaatt tgatttttaa attttaactt cacaattttt ttgctatcag
     3661 ataaaatttt ttttaaaaat attttcagat gacaagtttg tacaaaaaag ttgaacgaga
     3721 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     3781 ataatactgt aaaacacaac atatgcagtc actatgaatc aactacttag atggtattag
     3841 tgacctgtag aattcgagct ctagagctgc agggcggccg cgatatcccc tatagtgagt
     3901 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     3961 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     4021 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     4081 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     4141 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     4201 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     4261 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     4321 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     4381 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     4441 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     4501 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     4561 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     4621 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     4681 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     4741 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     4801 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     4861 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     4921 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     4981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     5161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     5401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5641 acatgtt
//
