LOCUS       sequence-9229-ex        3761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3761)
  TITLE     Forth generation Seydoux lab vectors
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCM1.107
FEATURES             Location/Qualifiers
     source          1..3761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1806..1930
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2002..2021)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2003..2021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2026..2042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2026..2042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2144..2163)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2155..2964
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2222..2241)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3111..3699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3600..3619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgat atataaaaaa actagttgaa aaagtttgtc
      721 accgaagata cgcccggtca gagacaaatg gcactttgtc gactggatac catgtcactt
      781 tggatacttt gtccccaaaa ataccttgcc accctaaaag tttgtcccct cggaatgagg
      841 gccagttacc agtggccata atcgtgtgat tattatttgg gatgttcgaa gtgtttgctc
      901 cgccctttct ggatcacaat agacaattgc ttcttctact tcttgaatgt ctcataattc
      961 ttcttatttt tatctaattt attgcttctc ctttctattt tgtatattaa ctcccaaaaa
     1021 cgcccattct tccccaaaac catctaaaac ttaccaatat ttcccgatac ccctgacagg
     1081 ttatcctgaa taatgattgt gattctcaaa tttcttctaa tcccgttaat tccccaaata
     1141 ttcgaatgta actttttttg gaattttttt gttttctagt ttataaataa acaatatgtt
     1201 tgaaaaacaa tcgtaatttg cgttttagat ggttaaacgg taggaagctt aagcttccta
     1261 caacatccaa atacaatgca atattttctg aaattctaaa tatcaaaaca tcatccggaa
     1321 tatggagatt taatgcaact ctatagaggt ttatggggac ttagtcttct ggaaattgaa
     1381 ttatgtgatt atgaattaat tttaaaaaaa ccaatcccaa tcatacgatc gtcgacgacg
     1441 atcgtccctc gcgtctcgcg agagccaact ggttatagct tttcgggagg tttaaatgtt
     1501 ttatcaattt tatacggtac ggtctggaat attccacaat tttttatctg ttttaacaga
     1561 gaatatctct ccatggagat gctaaaattt tgagagataa ataagcaaat cgcattgttt
     1621 gaaaatttca atttggatac ggtagacaat gacaaagtac gcaaacacca gagtatgtga
     1681 cacactttcg tcattctcaa attcaatttg aaatattatt ttcttattcc tattctctat
     1741 tttctgtgaa ttgatagttt ttctataagt aaccattcga ataaaattat caactaatcg
     1801 acatgacaag tttgtacaaa aaagttgaac gagaaacgta aaatgatata aatatcaata
     1861 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatgc
     1921 agtcactatg aatcaactac ttagatggta ttagtgacct gtagaattcg agctctagag
     1981 ctgcagggcg gccgcgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     2041 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     2101 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     2161 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     2221 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     2281 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     2341 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     2401 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     2461 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     2521 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     2581 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     2641 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     2701 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     2761 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     2821 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     2881 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     2941 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     3001 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     3061 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3241 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     3301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3481 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3721 ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
