LOCUS       sequence-92310-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insect expression vector for parallel SLIC cloning containing 
            N-terminal 10x His and SumoStar tags. C-terminal tags can be added 
            during SLIC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy44
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..103
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             join(107..112,113..136,137..172,173..196)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             200..244
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             251..328
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             333..368
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa gagcatcatc atcatcacca ccaccaccac cactgaagcg cttggagcca
      121 cccgcagttc gaaaaaggtg gaggttctgg cggtggatcg ggaggttcag cgtggagcca
      181 cccgcagttc gagaaataga aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      241 cagctagatg aagcggcggt ggaagaaaaa ctttatcgcc gtgtccgccg ccaaccggtt
      301 caagaagatc agcagctctg gcgccctgct gagaggacca ggtggacccc cggctgatcg
      361 acggcaaatg aggcagcggc aagatcctgc acaaccagaa cgtgaacagc tggggcccca
      421 tcaccgtgac ccctaccacc gacggcggcg agacaagatt cgacggccag atcatcgtgc
      481 agatggaaaa cgaccccgtg gtggccaagg ccgctgccaa tctggccggc aagcacgccg
      541 agagcagcgt ggtggtgcag ctggacagcg acggcaacta ccgggtggtg tacggcgacc
      601 ccagcaagct ggacggcaag ctgcggtggc agctggtggg acacggcagg gaccacagcg
      661 agacaaacaa cacccggctg agcggctaca gcgccgacga gctggccgtg aagctggcca
      721 agttccagca gagcttcaac caggccgaga acatcaacaa caagcccgac cacatcagca
      781 tcgtgggctg cagcctggtg tccgacgaca agcagaaggg cttcggccac cagttcatca
      841 acgctatgga cgccaacggc ctgcgggtgg acgtgtccgt gcggtctagc gaactggccg
      901 tggacgaggc cggcagaa
//
