LOCUS       sequence-92315-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression vector for parallel SLIC cloning containing 
            N-terminal 6x His and GST tags. C-terminal tags can be added during 
            SLIC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy48
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..43
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             join(47..52,53..76,77..112,113..136)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             140..184
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             191..268
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             275..310
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
ORIGIN
        1 gggaatataa gagcatcatc atcatcacca ccaccaccac cactgaagcg cttggagcca
       61 cccgcagttc gaaaaaggtg gaggttctgg cggtggatcg ggaggttcag cgtggagcca
      121 cccgcagttc gagaaataga aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      181 cagctagatg aagcggcggt ggaagaaaaa ctttatcgcc gtgtccgccg ccaaccggtt
      241 caagaagatc agcagctctg gcgccctgct gtgagaggac caggtggacc cccggctgat
      301 cgatggcaaa tgaggcagcg gcaagatcct gcacaaccag aacgtgaaca gctggggccc
      361 catcaccgtg acccctacca ccgacggcgg cgagacaaga ttcgacggcc agatcatcgt
      421 gcagatggaa aacgaccccg tggtggccaa ggccgctgcc aatctggccg gcaagcacgc
      481 cgagagcagc gtggtggtgc agctggacag cgacggcaac taccgggtgg tgtacggcga
      541 ccccagcaag ctggacggca agctgcggtg gcagctggtg ggacacggca gggaccacag
      601 cgagacaaac aacacccggc tgagcggcta cagcgccgac gagctggccg tgaagctggc
      661 caagttccag cagagcttca accaggccga gaacatcaac aacaa
//
