LOCUS       sequence-92316-e        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1-969aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Chan NC, den Besten W, Sweredoski MJ, Hess S, Deshaies RJ, Chan DC
  TITLE     Degradation of the Deubiquitinating Enzyme USP33 is Mediated by p97 
            and the Ubiquitin Ligase HERC2.
  JOURNAL   J Biol Chem. 2014 May 22. pii: jbc.M114.569392.
  PUBMED    24855649
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 22. pii: jbc.M114.569392."
COMMENT     Sequence Label: pcDNA SF-HERC2 F1
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             113..178
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             188..211
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcac gcgtccacca tggactacaa
      121 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaaggg
      181 gtcggcctgg agccacccgc agttcgaaaa aggatccact agtccagtgt ggtggaattc
      241 tgcagatatc cagcacagtg gcggccgctc gagtatgccc tctgaatctt tctgtttggc
      301 tgcccaggct cgcctcgact ccaaatggtt gaaaacagat atacagcttg cattcacaag
      361 agatgggctc tgtggtctgt ggaatgaaat ggttaaagat ggagaaattg tatacactgg
      421 aacagaatca acccagaacg gagagctccc tcctagaaaa gatgatagtg tcgaaccaag
      481 tggaacaaag aaagaagatc tgaatgacaa agagaaaaaa gatgaagaag aaactcctgc
      541 acctatatat agggccaagt caattctgga cagctgggta tggggcaagc aaccagatgt
      601 gaatgaactg aaggagtgtc tttctgtgct ggttaaagag cagcaggccc tggccgtcca
      661 gtcagccacc accaccctct cagccctgcg actcaagcag aggctggtga tcttggagcg
      721 ctatttcatt gccttgaata gaaccgtttt tcaggagaat gtcaaagtta agtggaaaag
      781 cagcggtatt tctctgcctc ctgtggacaa aaaaagttcc cggcctgcgg gcaaaggtgt
      841 ggaggggctc gccagagtgg gatcccgagc ggcgctgtct tttgcctttg ccttcctgcg
      901 cagggcctgg cgatcaggcg aggatgcgga cctctgcagt gagctgttgc aggagtccct
      961 ggacgccctg cgagcacttc ccgaggcctc gctctttgac gagagcaccg tgtcctctgt
     1021 gtggctggag gtggtgga
//
